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1.2.1.25: branched-chain alpha-keto acid dehydrogenase system

This is an abbreviated version!
For detailed information about branched-chain alpha-keto acid dehydrogenase system, go to the full flat file.

Word Map on EC 1.2.1.25

Reaction

3-methyl-2-oxobutanoate
+
CoA
+
NAD+
=
2-methylpropanoyl-CoA
+
CO2
+
NADH
+
H+

Synonyms

2-oxoisovalerate dehydrogenase, alpha-ketoisovalerate dehydrogenase, BCKD, BCKD complex, BCKDalpha, BCKDbeta, BCKDC, BCKDH, BCKDHA, BCKDHB, bkdA, BKDH, branched chain alpha-keto acid dehydrogenase complex, branched chain keto-acid dehydrogenase, branched-chain alpha-keto acid dehydrogenase complex, branched-chain alpha-ketoacid dehydrogenase, branched-chain alpha-ketoacid dehydrogenase complex, branched-chain alpha-ketoacid dehydrogenase complex E1alpha subunit, branched-chain ketoacid dehydrogenase, branched-chain-keto-acid dehydrogenase alpha, branched-chain-keto-acid dehydrogenase beta, dehydrogenase, 2-oxoisovalerate (acylating), E1alpha subunit, E1beta subunit, mitochondrial branched-chain keto-acid dehydrogenase, More

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.25 branched-chain alpha-keto acid dehydrogenase system

Engineering

Engineering on EC 1.2.1.25 - branched-chain alpha-keto acid dehydrogenase system

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
improvement of the production of short branched-chain acyl-CoAs for avermectin biosynthesis by functional expression of the branched chain alpha-keto acid dehydrogenase complex (BKDH) from Streptomyces avermitilis, systematic optimization by selectively regulating individual subunit expression in Escherichia coli, codon optimization, method, overview. Synergic synthesis pathway of avermectin precursors. Codon optimization could improve the expression of BkdH and LpdA1 from pRB1a, we further coexpressed the bkdF and bkdG with wild-type or codon optimized bkdH and lpdA1 by constructing vectors pRB01 (wild-type) and pRB02 (optimized). The bkdF-bkdG and bkdH-lpdA1 gene clusters were placed under the control of two independent araBAD promoters in pRB1a. Genes bkdF and bkdG are respectively inserted close to the araBAD promoters so as to enhance their expression