1.2.1.26: 2,5-dioxovalerate dehydrogenase

This is an abbreviated version!
For detailed information about 2,5-dioxovalerate dehydrogenase, go to the full flat file.

Reaction

Synonyms

2-oxoglutarate semialdehyde dehydrogenase, AbKGSADH, aldehyde dehydrogenase, ALDH, alpha-ketoglutarate semialdehyde dehydrogenase, alpha-ketoglutarate-semialdehyde dehydrogenase, alpha-ketoglutaric semialdehyde dehydrogenase, alpha-KGSA dehydrogenase, alphaKGSA dehydrogenase, alphaKGSADH, araE, DopDH, KGSADH, KGSADH-I, KGSADH-II, KGSADH-III, More, SSO3117, ycbD protein

ECTree

     1 Oxidoreductases

         1.2 Acting on the aldehyde or oxo group of donors

             1.2.1 With NAD⁺ or NADP⁺ as acceptor

                1.2.1.26 2,5-dioxovalerate dehydrogenase

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Results	in table
5	Activating Compound
933	AA Sequence
5	Application
1	CAS Registry Number
8	Cloned(Commentary)
16	Cofactor
1	Crystallization (Commentary)
5	General Information
3	Inhibitors
21	kcat/KM [mM/s]
56	KM Value [mM]
1	Metals/Ions
9	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
26	Organism
16	Pathway
4	PDB ID
6	pH Optimum
2	pH Range
18	Protein Variants
9	Purification (Commentary)
1	Reaction
3	Reaction Type
18	Reference
2	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
55	Substrates and Products (Substrate)
11	Subunits
49	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Stability [°C]
54	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.2.1.26 - 2,5-dioxovalerate dehydrogenase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant enzyme in apoform and in complex with NAD+, sitting-drop vapor diffusion method, mixing of 0.001 ml of 60 mg/ml protein in 40 mM Tris-HCl pH 8.0 with 0.001 ml of reservoir solution containing 16% PEG 3350, 0.1 M sodium cacodylate, pH 6.5, and 0.2 M magnesium chloride hexahydrate, equilibration against 0.05 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.25 A and 2.30 A resolution, molecular replacement structure modeling using the structure of succinic semialdehyde dehydrogenase (SSADH) from Homo sapiens (PDB ID 2W8R) as a search model for the apoenzyme, and the crystal structure of the apoform of AbKGSADH as a template for the NAD+-complexed AbKGSADH structure

Azospirillum brasilense

Q1JUP4

763717