1.2.1.27: methylmalonate-semialdehyde dehydrogenase (CoA-acylating)

This is an abbreviated version!
For detailed information about methylmalonate-semialdehyde dehydrogenase (CoA-acylating), go to the full flat file.

Word Map on EC 1.2.1.27

Reaction

2-Methyl-3-oxopropanoate
+
CoA
+
H2O
+
NAD+
=
propanoyl-CoA
+
HCO3-
+
NADH

Synonyms

Aldh6a1, dddC, FG99_15390, KES23460, malonate-semialdehyde dehydrogenase, methylmalonate semialdehyde dehydrogenase, methylmalonate semialdehyde dehydrogenase protein, methylmalonate-semialdehyde dehydrogenase, methylmalonic acid semialdehyde dehydrogenase, MMSADH, MMSDH, MSDH, NAD-dependent malonate-semialdehyde dehydrogenase, OdoMMSDH, OsALDH6, PA3570, SSO1218

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.27 methylmalonate-semialdehyde dehydrogenase (CoA-acylating)

Crystallization

Crystallization on EC 1.2.1.27 - methylmalonate-semialdehyde dehydrogenase (CoA-acylating)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour diffusion, crystals diffract to 2.5 A resolution
-
methylmalonate semialdehyde dehydrogenase in binary complex with NAD+, showing that the nicotinamide ring is well defined in the electron density due to direct and H2O-mediated hydrogen bonds with the carboxamide and that a conformational isomerization of the NMNH is possible in MSDH
purified recombinant His6-tagged enzyme, mixing of 0.001 ml of protein solution containing 32.2 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 1 mM DTT, with 0.001 ml of reservoir solution consisting of 21% PEG 3350 and 0.2 M potassium sodium tartrate, pH 7.5, at 20°C, 2 days, X-ray diffraction sructure determination and analysis at 2.9 A resolution, Among the twelve molecules in the asymmetric unit, six subunits complexed with NAD+
purified recombinant His-tagged enzyme, microseeding, mixing of 150 nl of 5 mg/ml protein in 40 mM Tris Cl, 150 mM NaCl, 2 mM KCl, and TBS, pH 8, with 150 nl of reservoir solution containing 23.7% w/v PEG 3350, 0.208 M trisodium citrate, 0.1 M Bis-Tris propane, pH 7.55, and equilibration against 0.05 ml of reservoir solution, at 8°C, X-ray diffraction structure determination and analysis at 2.95 A resolution, molecular replacement and modeling using PDB entry 4zz7 as a template