1.2.1.3: aldehyde dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about aldehyde dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.2.1.3

Reaction

an aldehyde
+
NAD+
+
H2O
=
a carboxylate
+
NADH
+
H+

Synonyms

ADH, AdhE, AHD-M1, ALD1A1, ALD4, ALDDH, aldehyde dehydrogenase, aldehyde dehydrogenase (NAD), aldehyde dehydrogenase 1, aldehyde dehydrogenase 1A1, aldehyde dehydrogenase 1A3, aldehyde dehydrogenase 1B1, aldehyde dehydrogenase 2, aldehyde dehydrogenase 3A1, aldehyde dehydrogenase 7A1, aldehyde dehydrogenase class 1, aldehyde dehydrogenase type 2, Aldehyde dehydrogenase [NAD+], Aldehyde dehydrogenase, cytosolic, Aldehyde dehydrogenase, microsomal, aldehyde dehydrogenase-2, aldehyde dehydrogenase7, aldehyde:NAD+ oxidoreductase, ALDH, ALDH 2, ALDH I, ALDH II, ALDH-2, ALDH-E1, ALDH-E2, ALDH1, ALDH1-NL, ALDH1A1, Aldh1a2, Aldh1a3, Aldh1a7, Aldh1b1, ALDH2, ALDH2(1), ALDH2(2), ALDH2(3), ALDH22A1, ALDH2B8, ALDH2C4, ALDH3A1, ALDH3A2, Aldh3b1, ALDH3H1, ALDH5A, ALDH7, Aldh7a1, Aldh8a1, ALDHI, ALDHX, ALHDII, Allergen Alt a 10, aryl-aldehyde dehydrogenases, BADH, benzaldehyde dehydrogenase, Brassica turgor-responsive/drought-induced gene 26 protein, Bt-Aldh, Btg-26, class 1 aldehyde dehydrogenase, class 2 aldehyde dehydrogenase, class I ALDH, CoA-independent aldehyde dehydrogenase, coniferyl-aldehyde dehydrogenase, Cphy_1178, ETA-crystallin, FeaB-K-12, gamma-aminobutyraldehyde dehydrogenase, hALDH2, irreversible NAD+-dependent aldehyde dehydrogenase, K(+)-ACDH, K(+)-activated acetaldehyde dehydrogenase, KGSADH, m-methylbenzaldehyde dehydrogenase, Matured fruit 60 kDa protein, MF-60, Mg(2+)-ACDH, Mg(2+)-activated acetaldehyde dehydrogenase, mitochondrial aldehyde dehydrogenase, NAD+-dependent aldehyde dehydrogenase, NAD+-dependent ALDH, NAD+-linked aldehyde dehydrogenase, NAD+-pimelic semialdehyde-dependent aldehyde dehydrogenase, NAD+-specific ALDH, NAD-aldehyde dehydrogenase, NAD-dependent 4-hydroxynonenal dehydrogenase, NAD-dependent aldehyde dehydrogenase, NAD-linked aldehyde dehydrogenase, Non-lens ALDH1, p-ALDH2, P51, phenylacetaldehyde dehydrogenase, PhnN, phnY, phosphonoacetaldehyde dehydrogenase, phosphonoacetaldehyde oxidase, PM-ALDH9, PnAA dehydrogenase, propionaldehyde dehydrogenase, PuuC, R-aminobutyraldehyde dehydrogenase, RALDH, RALDH(II), RalDH1, Retinal dehydrogenase, retinal dehydrogenase type I, sALDH, salvery aldehyde dehydrogenase, ThnG, Turgor-responsive protein 26G, yALDH, YneI

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.3 aldehyde dehydrogenase (NAD+)

Purification

Purification on EC 1.2.1.3 - aldehyde dehydrogenase (NAD+)

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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
5'-AMP-Sepharose 4B affinity column chromatography, gel filtration
-
5'-AMP-Sepharose 4B affinity column, 95% purity
ammonium sulfate precipitation, column chromatography, and gel filtration
-
ammonium sulfate precipitation, column chromatography, gel filtration
-
by p-hydroxyacetophenone affinity chromatography; by p-hydroxyacetophenone affinity chromatography and gel filtration
-
chelating Sepharose column chromatography, gel filtration
-
corneal epithelial extracts are prepared
-
cytoplasmic enzyme
-
DEAE MacroPrep column chromatography
-
DEAE-Sepharose chromatography
-
enzyme from promotion phase of hepatocarcinogenesis and phenobarbital-inducible enzyme
-
enzyme I and II
-
enzyme purified from the mitochondrial fraction by p-hydroxyacetophenone affinity chromatography
-
glutathione Sepharose 4B affinity chromatography
-
His Gravi-Trap column chromatography
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His-tag binding column chromatography
-
HisTrap column chromatography, Q-sepharose column chromatography, and S200 gel filtration
A9KN57
immobilized metal-ion affinity column chromatography
-
isoenzyme ALDH1 and ALDH2 from normal livers, ALDH1 from the atypical phenotype
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isoenzyme ALDH1 and ALDH2 from normal livers, ALDH1 from the atypical phenotype; isoenzyme E3
-
membrane-bound ALDH, ALDH-I: soluble high Km ALDH, and ALDH-II: low Km ALDH
-
microsomal enzyme
-
native and mutant enzyme S74A
-
Ni-affinity chromatography
Ni-NTA affinity column chromatography
-
Ni-NTA chromatography and Sephacryl S200 gel filtration
-
Ni-NTA column chromatography
Ni-NTA column chromatography and Superdex 75 gel filtration
-
Ni-NTA resin column chromatography and Superdex 75 gel filtration
-
Ni-NTA resin column chromatography, and gel filtration
-
Ni-NTA-HP resin column chromatography
-
Ni2+-NTA resin column chromatography
-
Ni2+-Sepharose column chromatography and Superdex S200 gel filtration
-
nickel affinity column chromatography, and Superdex 200 gel filtration
nickel chelating Sepharose column chromatography
-
nickel column chromatography
p-hydroxyacetophenone Sepharose affinity column
-
partial, phenobarbital-induced enzyme
-
rapid purification, K+-activated enzyme
-
recombinant ALDH3A1 is isolated with Ni-NTA Fast Start Kit
-
recombinant wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, tag cleavage by TEV protease, dialysis, and gel filtration
-
Sephacryl S-200 gel filtration, DEAE-Sepharose anion-exchange column chromatography, and Affi-Gel blue column chromatography
-
two chromatography steps to homogeneity
two chromatography steps, homogeneous on SDS-PAGE
-