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1.2.1.36: retinal dehydrogenase

This is an abbreviated version!
For detailed information about retinal dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.36

Reaction

retinal
+
NAD+
 +
H2O
=
retinoate
 +
NADH
 +
H+

Synonyms

aldehyde dehydrogenase 1, aldehyde dehydrogenase 1A1, aldehyde dehydrogenase 1A2, aldehyde dehydrogenase 1A3, ALDH 1A1, ALDH 1A2, ALDH class 1, ALDH-1A1, ALDH-3A1, ALDH1, ALDH1A1, Aldh1a2, Aldh1a3, BcALDH, cytosolic retinal dehydrogenase, dehydrogenase, retinal, dehydrogenase/reductase member 3, DHRS3, ETA-crystallin, More, NAD+-dependent aldehyde dehydrogenase, NAD-dependent retinal dehydrogenase, NAD-dependent retinal dehydrogenases, PB-RALDH, phenobarbital-induced aldehyde dehydrogenase, RALDH, RALDH-1, RALDH-3, RalDH1, RALDH1A1, RALDH2, RALDH3, Raldh4, RDH12, RDH13, RDH5, retialdehyde dehydrogenase, Retinal dehydrogenase, retinal dehydrogenase 1, retinal dehydrogenase 2, retinal dehydrogenase 3, retinal dehydrogenase 4, retinal dehydrogenase 5, retinal dehydrogenase type-2, retinal dehydrogenase-2, retinal dehydrogenase/reductase, retinal short-chain dehydrogenase/reductase, retinalaldehyde dehydrogenase 2, retinaldehyde dehydrogenase, retinaldehyde dehydrogenase 1, retinaldehyde dehydrogenase 2, retinaldehyde dehydrogenase 3, retinaldehyde dehydrogenase type II, retinaldehyde dehydrogenase type-1, retinaldehyde dehydrogenase-2, retinaldehyde dehydrogenases, retinol dehydrogenase 13, retSDR1, testis-specific aldehyde dehydrogenase, thyroid-hormone-binding protein, type 2 retinaldehyde dehydrogenase, xCTBP, xCTBP/xALDH1, zRalDH

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.36 retinal dehydrogenase

Cloned

Cloned on EC 1.2.1.36 - retinal dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into the pET28a vector. Expressed in Sf9 cells as a fusion with the C-terminal His6 tag
-
expressed in COS-1 cells
-
expressed in Escherichia coli BL21(DE3) cells as His-tagged enzyme
expressed in Escherichia coli C41(DE3) cells
expressed in Escherichia coli Rosetta(DE3) cells
expressed in HEK-293 cells
expression in Escherichia coli
-
expression in Escherichia coli BL21 (DE3) as His-tag fusion protein
-
expression of isozyme type-1 in Escherichia coli strain BL21(DE3) as N-terminally fusion protein to glutathione-S-transferase
-
expression of isozyme type-2 in Escherichia coli strain BL21(DE3) as N-terminally fusion protein to glutathione-S-transferase
-
expression of xCTBP/xALDH1 in Escherichia coli
-
functional expression of wild-type and chimeric mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene Aldh1a1, quantitative reverse transcription PCR enzyme expression analysis
gene ALDH1A1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLys
gene ALDH1A1, recombinant expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain C41(DE3)
gene Aldh1a2, comprises 14 exons that are divided by 13 introns spanning more than 50 kb of genomic DNA, and it is localized on chromosome 9. CpG methylation in the Aldh1a2 promoter region inhibits Sp1-dependent Aldh1a2 promoter activation
gene ALDH1A2, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain C41(DE3)
into the cloning vector pGEM-T Easy and subcloned into the expression vector pET14b
into the pGEM-T easy vector, 396-1002
into the pGEM-T easy vector, bp 1488-2079
into the pGEM-T easy vector, bp 935-1485
into the pKO-V901 plasmid
-
into the vector pET28a for expression in Escherichia coli BL21DE3 cells
ligated into a pCMV5 expression vector and overexpressed in HEK-293 cells
-
overexpression of wild-type and mutant isozyme type-2 in Escherichia coli strain Bl21(DE3)
-
recombinant H1299 cells expressing C302A, E107G, and I304K mutant forms of ALDH1A1 lack aldehyde-dehydrogenase activity
recombinant in Escherichia coli (pLysS cells)
-
recombinant in Escherichia coli BL21(DE3)
recombinant tagged enzyme expression in Escherichia coli strain ER2566