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1.2.1.42: hexadecanal dehydrogenase (acylating)

This is an abbreviated version!
For detailed information about hexadecanal dehydrogenase (acylating), go to the full flat file.

Word Map on EC 1.2.1.42

Reaction

hexadecanal
+
CoA
+
NAD+
=
hexadecanoyl-CoA
+
NADH
+
H+

Synonyms

ACIAD3383, Acr1, AtFAR2, CoA-acylating aldehyde dehydrogenase, coenzyme A-acylating aldehyde dehydrogenase, dehydrogenase, hexadecanal (acylating), FACoAR, FAR, FAR1, FAR2, FAR6, fatty acid reductase, fatty acyl-CoA reductase, MS2, PduP

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.42 hexadecanal dehydrogenase (acylating)

Purification

Purification on EC 1.2.1.42 - hexadecanal dehydrogenase (acylating)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
alcohol-generating and aldehyde-generating acyl-CoA reductases, Blue A agarose, gel filtration, hexadecanoyl-CoA affinity chromatography
-
amylose column chromatography, metal affinity column chromatography, and G25 Sephadex gel filtration
Marinobacter nauticus
-
Blue A agarose, Sephacryl S-100, palmitoyl-CoA agarose
-
heating, ammonium sulfate, gel filtration
-
HisTrap column chromatography and Ni2+ resin column chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography
solubilization from microsomal preparation with 20% glycerol and 3 M NaCl, affinity chromatography
-