1.2.1.46: formaldehyde dehydrogenase

This is an abbreviated version!
For detailed information about formaldehyde dehydrogenase, go to the full flat file.

Reaction

Synonyms

ADH3, dehydrogenase, formaldehyde, DL-FDH, dlFalDH, dye-linked formaldehyde dehydrogenase, FADH, FALDH, FdDH, FDH, FDH1, fdhA, FldDH, Fldh1, formaldehyde dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase, glutathione-independent FDH, glutathione-independent formaldehyde dehydrogenase, GSH-FDH/ADH, NAD+- and glutathione-dependent formaldehyde dehydrogenase, NAD+- and glutathione-independent formaldehyde dehydrogenase, NAD+- and glutathione_dependent formaldehyde dehydrogenase, NAD+-dependent formaldehyde dehydrogenase, NAD-dependent formaldehyde dehydrogenase, NAD-linked formaldehyde dehydrogenase

ECTree

     1 Oxidoreductases

         1.2 Acting on the aldehyde or oxo group of donors

             1.2.1 With NAD⁺ or NADP⁺ as acceptor

                1.2.1.46 formaldehyde dehydrogenase

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Results	in table
6	Activating Compound
1863	AA Sequence
14	Application
1	CAS Registry Number
9	Cloned(Commentary)
40	Cofactor
4	Crystallization (Commentary)
10	Disease/ Diagnostics
2	Expression
8	General Information
6	General Stability
39	Inhibitors
6	kcat/KM [mM/s]
13	Ki Value [mM]
38	KM Value [mM]
1	Localization
18	Metals/Ions
14	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
115	Organism
6	Pathway
2	PDB ID
14	pH Optimum
3	pH Range
4	pH Stability
4	Protein Variants
16	Purification (Commentary)
4	Reaction
6	Reaction Type
52	Reference
9	Source Tissue
15	Specific Activity [micromol/min/mg]
6	Storage Stability
95	Substrates and Products (Substrate)
23	Subunits
63	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Range [°C]
4	Temperature Stability [°C]
5	Turnover Number [1/s]

Cloned

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Cloned on EC 1.2.1.46 - formaldehyde dehydrogenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

construction of a recombinant yeast clone originated from the recipient strain of thermotolerant methylotrophic yeast Hansenula polymorpha which is a NAD+- and glutathione dependent FdDH over-producer

Ogataea angusta

-

701261

expressed in Escherichia coli

Pseudomonas putida

-

288253

expressed in Escherichia coli BL21(DE3) cells

Escherichia coli

-

762628

expressed in Escherichia coli BL21-Codon-Plus(DE3)-RIL cells

Bacillus subtilis

-

762739

FLD1 gene with its own promoter is recloned into LEU2-containing integrative plasmid pYT1 devoid of the autonomously replicating sequence to be used for multicopy integration of the gene into chromosome of the recipient strain NCYC 495

Ogataea angusta

-

686818

FLD1 gene with the own promoter inserted into the integrative plasmid pYT1 containing LEU2 gene of Saccharomyces cerevisiae (as a selective marker). The constructed vector is used for multi-copy integration of the target gene into genome by transformation of the recipient cells of the strain NCYC 495

Ogataea angusta

-

685697

for expression in Escherichia coli BL21 cells

Homo sapiens

-

667724

gene fdhA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)/pG-TF2

Pseudomonas aeruginosa

Q9HTE3

741521

recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)/pG-TF2. 50% of the recombinant proteins are localized in the supernatant fraction of cell-free extract, suggesting that coexpression with chaperones GroES, GroEL, and Tig has significantly improved the solubilization of Pseudomonas aeruginosa FDH in Escherichia coli

Pseudomonas aeruginosa

-

743745