1.2.1.48: long-chain-aldehyde dehydrogenase

This is an abbreviated version!
For detailed information about long-chain-aldehyde dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.48

Reaction

a long-chain aldehyde
+
NAD+
+
H2O
=
a long-chain carboxylate
+
NADH
+ 2 H+

Synonyms

ALDH10, ALDH3A2, Aldh3b1, ALDH3B2, ALDH3B3, Bt-Aldh, dehydrogenase, long-chain aliphatic aldehyde, FALDH, fatty aldehyde dehydrogenase, fatty aldehyde:NAD+ oxidoreductase, long-chain aldehyde dehydrogenase, long-chain fatty aldehyde dehydrogenase, long-chain-aldehyde dehydrogenase, membrane-bound fatty aldehyde dehydrogenase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.48 long-chain-aldehyde dehydrogenase

Purification

Purification on EC 1.2.1.48 - long-chain-aldehyde dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
crude enzyme solution is applied to an anionic exchange column and eluted by linearly increasing the NaCl concentration from 0-1.0 M in 50 mM Tris-HCl at pH 7.0
including chromatography in a Resource Q column, recombinant enzyme from Escherichia coli also purified
-
recombinant His6-FADH purified by anion exchange and metal-chelate chromatography, to 90-95% purity
-
recombinant Strep-tagged enzyme from Escherichia coli by affinity chromatography and gel filtration
Strep-tag affinity chromatography and AMP-Sepharose column chromatography
-
using chromatography on columns consisting of omega-aminohexyl-agarose and 5'-AMP-Sepharose 4B
-