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1.2.1.70: glutamyl-tRNA reductase

This is an abbreviated version!
For detailed information about glutamyl-tRNA reductase, go to the full flat file.

Word Map on EC 1.2.1.70

Reaction

L-glutamate 1-semialdehyde
+
NADP+
 +
tRNAGlu
=
L-glutamyl-tRNAGlu
 +
NADPH
 +
H+

Synonyms

AtHEMA1, EC 2.7.2.13, GluRS, glutamate tRNA reductase, glutamate-specific tRNA reductase, glutamyl transfer RNA reductase, glutamyl-tRNA reductase, GluTR, GluTR1, GTR, GtrR, hemA, HEMA1, HEMA2, reductase, glutamyl-transfer ribonucleate, ZjGluTR

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.70 glutamyl-tRNA reductase

Cloned

Cloned on EC 1.2.1.70 - glutamyl-tRNA reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AtHemA1 overexpression in Nicotiana tabacum cv Samsun NN using Agrobacterium tumefaciens and Arabidopsis thaliana by transformation
-
DNA and amino acid sequence determination and analysis, phylogenetic tree
-
expressed in Escherichia coli BL21 cells
-
expression as His-tag fusion protein in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli as a fusion protein with glutathione S-transferase
expression in Escherichia coli BL21
-
expression plasmid pBKCwt overexpression of a 6*His-tagged enzyme
-
gene HEMA1, recombinant co-overexpression of N-terminally His6-tagged FLUTPR and GluTRDD in Escherichia coli strain BL21(DE3)
hemA ligated into pUC19 vector with a Lys insert between Thr-2 and Leu-3 at N terminus, overexpression in Escherichia coli
-
His(6)-GluTR overexpressed in Escherichia coli BL21 (DE3)
-
N-terminal His6-tagged GluTR expressed in Escherichia coli
-
overexpression in Escherichia coli
recombinant enzyme expression in Rhodobacter sphaeroides strain DSM 5864, which synthesizes 5-aminolevulinic acid through the C-4 pathway and also has the genes coding for the enzymes of the C-5 pathway, except for glutamyl-tRNA reductase. Expression of the glutamyl-tRNA reductase gene from Rhodospirillum rubrum enabled the C-5 pathway in Rhodobacter sphaeroides to function upon assembling all the required genes
-
recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
wild-type GluTR and variants produced as N-terminal His6-fusion proteins