1.2.1.79: succinate-semialdehyde dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about succinate-semialdehyde dehydrogenase (NADP+), go to the full flat file.

Reaction

Synonyms

AbSSADH, ALDH21, all3556, ApSSADH, gabD, GabD1, NADP+-dependent SSADH, NADP+-dependent succinic semialdehyde dehydrogenase, NADP-dependent succinic semialdehyde dehydrogenase, PpSSALDH, slr0370, Sp2771, SpSSADH, SSADH, SSADH-II, SSALDH, SSO1842, succinic semialdehy de dehydrogenase, succinic semialdehyde dehydrogenase, SYNPCC7002_A2771, SySSADH

ECTree

     1 Oxidoreductases

         1.2 Acting on the aldehyde or oxo group of donors

             1.2.1 With NAD⁺ or NADP⁺ as acceptor

                1.2.1.79 succinate-semialdehyde dehydrogenase (NADP+)

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Results	in table
9	Activating Compound
4196	AA Sequence
1	Application
17	Cloned(Commentary)
34	Cofactor
11	Crystallization (Commentary)
5	Expression
26	General Information
1	IC50 Value
28	Inhibitors
33	kcat/KM [mM/s]
3	Ki Value [mM]
53	KM Value [mM]
2	Localization
10	Metals/Ions
11	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
34	Organism
3	Oxidation Stability
11	Pathway
4	PDB ID
11	pH Optimum
4	pH Range
1	pH Stability
1	Posttranslational Modification
35	Protein Variants
9	Purification (Commentary)
4	Reaction
31	Reference
6	Specific Activity [micromol/min/mg]
3	Storage Stability
64	Substrates and Products (Substrate)
17	Subunits
58	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
4	Temperature Stability [°C]
29	Turnover Number [1/s]

Purification

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Purification on EC 1.2.1.79 - succinate-semialdehyde dehydrogenase (NADP+)

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Saccharolobus solfataricus

Q97XA5

722211

nickel chelating Sepharose column chromatography and S200 16/60 gel filtration

Escherichia coli

P25526

710394

recombinant enzyme

Escherichia coli K-12

P25526

710394

recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography and ultrafiltration, followed by tag cleavage through PreScission protease in a batch and the protease resin is eliminated by centrifugation

Synechocystis sp. PCC 6803

Q55585

762707

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and dialysis, to homogeneity

Nostoc sp. PCC 7120 = FACHB-418

Q8YR92

763137

recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration

Synechococcus sp. PCC 7002

B1XMM6

743181

recombinant N-terminally His-tagged SySSADH from Escherichia coli strain B834(DE3) by nickel affinity chromatography, dialysis and cleavge fo the His-tag by tobacco etch mosaic virus protease, followed by another step of immobilized metal affinity chromatography and gel filtration. Recombinant His-tagged enzyme mutants from Escherichia coli strain coli BL21(DE3) by immobilized metal affinity chromatography and dialysis

Synechococcus sp.

B1XMM6

742844

through Ni2+ affinity column chromatography, followed by a Hi-Load Superdex S-75 26/60 column chromatography

Synechococcus sp.

B1XMM6

725838

using immobilized metal affinity chromatography, removal of N-terminal His tag, further purification by immobilized metal affinity chromatography and gel filtration using a Superdex 200 column

Synechococcus sp.

B1XMM6

725523