1.2.1.9: glyceraldehyde-3-phosphate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about glyceraldehyde-3-phosphate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.2.1.9

Reaction

D-glyceraldehyde 3-phosphate
+
NADP+
+
H2O
=
3-phospho-D-glycerate
+
NADPH
+ 2 H+

Synonyms

apo-GAPDH, apo-glyceraldehyde-3-phosphate dehydrogenase, dehydrogenase, glyceraldehyde phosphate (nicotinamide adenine dinucleotide phosphate), E268A-GAPN, GAP dehydrogenase, GAPDH, GAPDH (A4), GAPDHN, GAPN, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase (NADP), glyceraldehyde 3-phosphate:NADP+ reductase, non-phosphorylating, glyceraldehyde phosphate dehydrogenase (NADP), glyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase (NADP+), Glyceraldehyde-3-phosphate dehydrogenase [NADP+], glyceraldehyde-3-phosphate:NADP reductase, NADP+-dependent GAPDH, NADP+-dependent glyceraldehyde 3-phosphate dehydrogenase, NADP+-GAPDH, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase, NADP-glyceraldehyde phosphate dehydrogenase, NADP-glyceraldehyde-3-phosphate dehydrogenase, NADPH-dependent GAPDH, NADPH-glyceraldehyde-3-phosphate dehydrogenase, non phosphorylating glyceraldehyde-3-phosphate dehydrogenase, non-phosphorylating GAP dehydrogenase, Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase, non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase, non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase GAPN, non-phosphorylating NADP+-dependent Ga3PDHase, non-phosphorylating NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase, non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase, nonphosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, NP-Ga3PDHase, NP-GAPDH, OsALDH11, SSO3194, St-GAPN, STK_24770, triose phosphate dehydrogenase, Triosephosphate dehydrogenase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.9 glyceraldehyde-3-phosphate dehydrogenase (NADP+)

Cloned

Cloned on EC 1.2.1.9 - glyceraldehyde-3-phosphate dehydrogenase (NADP+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned and expressed in BL21 (DE3) CodonPlus using the pET expression vector system; expressed in Escherichia coli BL21(DE3) cells; expressed in Escherichia coli BL21(DE3) cells; expressed in Escherichia coli BL21(DE3) CodonPlus cells
EcoRI-digested genomic DNA into plasmid pUC18 for sequencing of gapN, into an expression vector for recombinant production of the enzyme in Escherichia coli; for expression in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) CodonPlus RIL cells
-
expressed in Escherichia coli C43 (DE3) cells; expression in Escherichia coli
-
expressed in Saccharomyces cerevisiae mutant AG2 with deletion of NAD+-dependent glycerol-3-phosphate dehydrogenase gene GPD2
-
expression by functional complementation of the Escherichia coli gap mutant W3CG
-
expression in Escherichia coli
expression in Escherichia coli BL-21
-
gene gapN, construction of the mutant Escherichia coli strain MG1655DELTAgapA::gapN using the fusion of the gapN gene with a chloramphenicol resistance cassette resulting in plasmid pTrcgapN
-
into the pET29 vector for expression in Escherichia coli BL21DE3 cells
-
into the pGEM-T easy vector for sequencing
overexpression in Escherichia coli BL21(DE3)-pLysS
-