glyceraldehyde-3-phosphate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about glyceraldehyde-3-phosphate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC


D-glyceraldehyde 3-phosphate
+ 2 H+


apo-GAPDH, apo-glyceraldehyde-3-phosphate dehydrogenase, dehydrogenase, glyceraldehyde phosphate (nicotinamide adenine dinucleotide phosphate), E268A-GAPN, GAP dehydrogenase, GAPDH, GAPDH (A4), GAPDHN, GAPN, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase (NADP), glyceraldehyde 3-phosphate:NADP+ reductase, non-phosphorylating, glyceraldehyde phosphate dehydrogenase (NADP), glyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase (NADP+), Glyceraldehyde-3-phosphate dehydrogenase [NADP+], glyceraldehyde-3-phosphate:NADP reductase, NADP+-dependent GAPDH, NADP+-dependent glyceraldehyde 3-phosphate dehydrogenase, NADP+-GAPDH, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase, NADP-glyceraldehyde phosphate dehydrogenase, NADP-glyceraldehyde-3-phosphate dehydrogenase, NADPH-dependent GAPDH, NADPH-glyceraldehyde-3-phosphate dehydrogenase, non phosphorylating glyceraldehyde-3-phosphate dehydrogenase, non-phosphorylating GAP dehydrogenase, Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase, non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase, non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase GAPN, non-phosphorylating NADP+-dependent Ga3PDHase, non-phosphorylating NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase, non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase, nonphosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, NP-Ga3PDHase, NP-GAPDH, OsALDH11, SSO3194, St-GAPN, STK_24770, triose phosphate dehydrogenase, Triosephosphate dehydrogenase


     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
       glyceraldehyde-3-phosphate dehydrogenase (NADP+)


Purification on EC - glyceraldehyde-3-phosphate dehydrogenase (NADP+)

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; HisTrap column chromatography and Superdex 200 gel filtration
cell-free extract preparation
DEAE Trisacryl column chromatography
heat precipitation and Superdex 200 gel filtration; Superdex 200 gel filtration; Superdex 200 gel filtration
heat treatment (85°C, 30 min), anion exchange chromatography and gel filtration
partial purification
recombinant and native GAPDH are purified to apparent homogeneity from Escherichia coli cells and Chlamydomonas reinhardtii, respectively
recombinant enzyme
using a Q-Sepharose HP and a MonoQ anion-exchange column