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1.2.5.2: aldehyde dehydrogenase (quinone)

This is an abbreviated version!
For detailed information about aldehyde dehydrogenase (quinone), go to the full flat file.

Word Map on EC 1.2.5.2

Reaction

an aldehyde
+
a quinone
+
H2O
=
a carboxylate
+
a quinol

Synonyms

ADH I, ADH IIB, ADH IIG, aldehyde dehydrogenase (acceptor), ALDH, BOH, dehydrogenase, aldehyde (acceptor), EC 1.2.99.3, FOE, formaldehyde-oxidizing enzyme, glucose sorbosone dehydrogenase, NAD+-independent, PQQ-containing alcohol dehydrogenase, PQQ-AldDH, PQQ-AlDH, pyrroloquinoline quinone-dependent aldehyde dehydrogenase, quinohemoprotein dehydrogenase, Swit_4395, tetrahydrofurfuryl alcohol dehydrogenase

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.5 With a quinone or similar compound as acceptor
                1.2.5.2 aldehyde dehydrogenase (quinone)

Purification

Purification on EC 1.2.5.2 - aldehyde dehydrogenase (quinone)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE Toyo-Pearl 650M column chromatography and Sephadex G-100 gel filtration
-
native enzyme 130fold from membranes of glycerol-grown cells by two different steps of anion exchange chromatography, solubilization with 0.1% Triton X-100 or Tween 20, copurification with a cytochrome c
-
native enzyme 211fold to homogeneity
-
native enzyme 37fold to near homogeneity by ammonium sulfate fractionation and anion exchange chromatography
-
native isozyme ADH I, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview
native isozyme ADH IIB, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview
native isozyme ADH IIG, a procedure using anion exchange and hydrophobic interaction chromatographies with elution buffers containing 50% glycerol or PEG 600, ultrafiltration, and gel filtration, overview
solubilization with Triton X-100, DEAE-cellulose, CM-cellulose
-
solubilization with Triton X-100/cetylpyridinium chloride, PEG precipitation, DEAE-cellulose, hydroxyapatite
-