1.2.7.B2: formaldehyde ferredoxin oxidoreductase
This is an abbreviated version!
For detailed information about formaldehyde ferredoxin oxidoreductase, go to the full flat file.
Word Map on EC 1.2.7.B2
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1.2.7.B2
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tungsten
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pyrococcus
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furiosus
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tungsten-containing
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hyperthermophilic
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archaeon
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tungstoenzymes
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iron-sulfur
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pterins
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homotetramer
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litoralis
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thermococcus
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two-electron
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glyceraldehyde-3-phosphate
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methyloversatilis
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wiv
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methylamine
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cubane
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pqq-dependent
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mxafi
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five-member
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molybdenum
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calvin
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nitrogenase
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universalis
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lake
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glutarate
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washington
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methylotrophic
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thermotolerans
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formic
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monoamine
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n-methylglutamate
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rhodocyclaceae
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two-subunit
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quinohemoprotein
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dye-mediated
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tungsten-dependent
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low-potential
- 1.2.7.B2
- tungsten
-
pyrococcus
- furiosus
-
tungsten-containing
-
hyperthermophilic
- archaeon
-
tungstoenzymes
-
iron-sulfur
- pterins
-
homotetramer
- litoralis
-
thermococcus
-
two-electron
- glyceraldehyde-3-phosphate
- methyloversatilis
- wiv
- methylamine
- cubane
-
pqq-dependent
-
mxafi
-
five-member
- molybdenum
-
calvin
- nitrogenase
- universalis
-
lake
- glutarate
-
washington
-
methylotrophic
- thermotolerans
-
formic
-
monoamine
- n-methylglutamate
- rhodocyclaceae
-
two-subunit
-
quinohemoprotein
-
dye-mediated
-
tungsten-dependent
-
low-potential
Reaction
Synonyms
FOR, formaldehyde ferredoxin oxidoreductase, formaldehyde:Fd oxidoreductase, formaldehyde:ferredoxin oxidoreductase, PF1203
ECTree
1.2.7.B2 formaldehyde ferredoxin oxidoreductaseAdvanced search results
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results (Metals Ions
Metals Ions on EC 1.2.7.B2 - formaldehyde ferredoxin oxidoreductase
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METALS and IONS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Calcium
contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Iron
Mo5+
molybdenum can be incorporated, Mo(V) signal is observed in electron paramagnetic resonance. Aldehyde oxidation activity correlates only with the residual tungsten content. This suggests that the Mo-containing enzymes are most likely inactive. An intracellular selection mechanism for tungstate and molybdate processing has to be present, since tungsten is found to be preferentially incorporated even under conditions with comparable intracellular concentrations of tungstate and molybdate
Tungsten
Iron
an electron transfer pathway is proposed that begins at the tungsten center, leads to the (4Fe-4S) cluster of formaldehyde ferredoxin oxidoreductase via one of the two pterins that coordinate the tungsten, and ends at the (4Fe-4S) cluster of ferredoxin. This pathway includes two residues that coordinate the (4Fe-4S) clusters, Cys287 of FOR and Asp14 of ferredoxin
Iron
contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Iron
the enzyme contains 16.0 gatom of iron per 280000 Da g of enzyme. Each subunit of dithionite reduced enzyme contains a magnetically isolated [4Fe-4S]1+ cluster that exists in both the S = 3/2 and S = 1/2 states
Tungsten
an electron transfer pathway is proposed that begins at the tungsten center, leads to the (4Fe-4S) cluster of formaldehyde ferredoxin oxidoreductase via one of the two pterins that coordinate the tungsten, and ends at the (4Fe-4S) cluster of ferredoxin. This pathway includes two residues that coordinate the (4Fe-4S) clusters, Cys287 of FOR and Asp14 of ferredoxin
Tungsten
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the enzyme from Pyrococcus furiosus uses exclusively tungsten to synthesize the catalytically active forms of formaldehyde ferredoxin oxidoreductase and active molybdenum- or vanadium-containing isoenzymes are not expressed when the cells are grown in the presence of these other metals
Tungsten
the tungsten in the active enzyme is a two-electron acceptor, W(VI/IV)
Tungsten
tungsten-containing enzyme. The enzyme contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Tungsten
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tungsten-dependent enzyme, no activity when tungsten is replaced by molybdenum
Tungsten
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tungsten-dependent enzyme. A mechanism is proposed in which the formaldehyde substrate binds directly to the tungsten ion. W(VI)=O then performs a nucleophilic attack on the formaldehyde carbon to form a tetrahedral intermediate. In the second step, which is calculated to be rate limiting, a proton is transferred to the second-shell Glu308 residue, coupled with a two-electron reduction of the tungsten ion
Tungsten
a W(VI) species with terminal sulfido or thiol ligands is proposed to be responsible for the catalytic activity in sulfide-activated samples of formaldehyde ferredoxin oxidoreductase
Tungsten
tungsten-containing enzyme. The enzyme contains 3.3 gatom of tungsten per 280000 Da g of enzyme
