1.3.1.103: 2-haloacrylate reductase
This is an abbreviated version!
For detailed information about 2-haloacrylate reductase, go to the full flat file.

Word Map on EC 1.3.1.103
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1.3.1.103
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2-chloroacrylate
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nadph-dependent
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synthesis
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dehalogenase
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organohalogen
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biotechnol
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unsaturated
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dl-2-haloacid
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herbicides
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lycopene
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analysis
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transhydrogenase
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burkholderia
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high-value
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cofactor-dependent
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pntab
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fluoroacetate
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costly
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halogen
- 1.3.1.103
- 2-chloroacrylate
-
nadph-dependent
- synthesis
-
dehalogenase
-
organohalogen
-
biotechnol
- unsaturated
-
dl-2-haloacid
- herbicides
- lycopene
- analysis
-
transhydrogenase
- burkholderia
-
high-value
-
cofactor-dependent
- pntab
- fluoroacetate
-
costly
-
halogen
Reaction
Synonyms
CAA43
ECTree
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Application
Application on EC 1.3.1.103 - 2-haloacrylate reductase
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analysis
synthesis
monitoring the production of (S)-2-chloropropionate from 2-chloroacrylate as a model system for monitoring NADPH availability. A phosphofructokinase pfkA pfkB double-deletion strain shows the highest yield of 2-chloropropionic acid product. The flux distribution of fructose-6-phosphate between glycolysis and the pentose phosphate pathway determines the amount of NAPDH available for reductive biosynthesis
analysis
monitoring the production of (S)-2-chloropropionate from 2-chloroacrylate as a model system for monitoring NADPH availability. The presence of transhydrogenase UdhA increases product yield and NADPH availability while the presence of transhydrogenase PntAB has the opposite effect. A maximum product yield of 1.4 mol product/mol glucose is achieved aerobically in a Pnt-deletion strain with UdhA overexpression, a 150% improvement over the wild-type strain
construction of a system for asymmetric reduction of 2-chloroacrylate to produce (S)-2-chloropropionate with recombinant Escherichia coli cells producing 2-haloacrylate reductase from Burkholderia sp. WS and an NADPH regeneration system. The system provided 37.4 g/l (S)-2-chloropropionate in more than 99.9%e.e. (S)-2-Chloropropionate is a synthetic intermediate for phenoxypropionic acid herbicides
synthesis
the enzyme is useful for the production of (S)-2-chloropropionate, which is used for the industrial production of aryloxyphenoxypropionic acid herbicides
synthesis
the enzyme is useful in the production of L-2-chloropropionate, a building block in the synthesis of aryloxyphenoxypropionate herbicides