1.3.1.104: enoyl-[acyl-carrier-protein] reductase (NADPH)
This is an abbreviated version!
For detailed information about enoyl-[acyl-carrier-protein] reductase (NADPH), go to the full flat file.
Reaction
Synonyms
enoyl-ACP reductase, enoyl-acyl carrier protein reductase, enoyl-[acyl carrier protein] (reduced nicotinamide adenine dinucleotide phosphate) reductase, ENR, ETR1, FabI, FabL, Fabl2, JamJ, NADPH 2-enoyl Co A reductase, NWMN_0881, YgaA
ECTree
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Engineering
Engineering on EC 1.3.1.104 - enoyl-[acyl-carrier-protein] reductase (NADPH)
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A95V
F204S
G23S
mutation increases the resistance of Staphylococcus aureus to triclosan by an order of magnitude
I193S
no shift in melting temperature in presence of both triclosan and NADP+
M99T
Y147H
mutant is resistant to antibiotic AFN-1252 and to triclosan, about 5% of wild-type catalytic activity. A strain expressing Y147H has a pronounced growth defect that is rescued by exogenous fatty acid supplementation
A95V
-
about 15% of wild-type activtiy, no inhibition by triclosan and no shift in melting temperature in presence of both triclosan and NADP+
-
F204S
-
no shift in melting temperature in presence of both triclosan and NADP+
-
I193S
-
no shift in melting temperature in presence of both triclosan and NADP+
-
M99T
Y147H
-
mutant is resistant to antibiotic AFN-1252 and to triclosan, about 5% of wild-type catalytic activity. A strain expressing Y147H has a pronounced growth defect that is rescued by exogenous fatty acid supplementation
-
additional information
replacement of amino acids 55-66 by CurF enoyl reductase cyclopropanase loop leads to gain-of-function activity as a cyclopropanase. With substrate 3-chloromethyl-crotonyl-[acyl-carrier-protein], the formation of both the reduced product and also the cyclopropanated productis detected at levels of about one-quarter of the total product
A95V
about 15% of wild-type activtiy, no inhibition by triclosan and no shift in melting temperature in presence of both triclosan and NADP+
F204S
no shift in melting temperature in presence of both triclosan and NADP+
mutant is resistant to antibiotic AFN-1252 but susceptible to triclosan. Strains expressing M99T exhibit normal growth, and the biochemical properties of the purified protein are indistinguishable from those of FabI
M99T
mutant shows a similar shift in melting temperature in presence of both triclosan and NADP+ as wild-type
-
mutant shows a similar shift in melting temperature in presence of both triclosan and NADP+ as wild-type
-
M99T
-
mutant is resistant to antibiotic AFN-1252 but susceptible to triclosan. Strains expressing M99T exhibit normal growth, and the biochemical properties of the purified protein are indistinguishable from those of FabI
-