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1.3.1.104: enoyl-[acyl-carrier-protein] reductase (NADPH)

This is an abbreviated version!
For detailed information about enoyl-[acyl-carrier-protein] reductase (NADPH), go to the full flat file.

Reaction

acyl-[acyl-carrier protein]
+
NADP+
=
trans-2,3-dehydroacyl-[acyl-carrier protein]
+
NADPH
+
H+

Synonyms

enoyl-ACP reductase, enoyl-acyl carrier protein reductase, enoyl-[acyl carrier protein] (reduced nicotinamide adenine dinucleotide phosphate) reductase, ENR, ETR1, FabI, FabL, Fabl2, JamJ, NADPH 2-enoyl Co A reductase, NWMN_0881, YgaA

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.104 enoyl-[acyl-carrier-protein] reductase (NADPH)

Engineering

Engineering on EC 1.3.1.104 - enoyl-[acyl-carrier-protein] reductase (NADPH)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D273A
about 55% of wild-type activity
D273N
about 70% of wild-type activity
K251A
activity similar to wild-type
K251R
activity similar to wild-type
A230T
-
triclosan-resistant mutant
D66Y
-
active-site mutant
F204S
F252I
-
triclosan-resistant mutant
G113V
-
triclosan-resistant mutant
G227C
-
triclosan-resistant mutant
G23S
mutation increases the resistance of Staphylococcus aureus to triclosan by an order of magnitude
H253P
-
triclosan-resistant mutant
I193S
no shift in melting temperature in presence of both triclosan and NADP+
K164R
-
active-site mutant
N220I
-
triclosan-resistant mutant
S166N
-
triclosan-resistant mutant
V241I
-
triclosan-resistant mutant
Y123H
-
triclosan-resistant mutant
Y147H
mutant is resistant to antibiotic AFN-1252 and to triclosan, about 5% of wild-type catalytic activity. A strain expressing Y147H has a pronounced growth defect that is rescued by exogenous fatty acid supplementation
A95V
-
about 15% of wild-type activtiy, no inhibition by triclosan and no shift in melting temperature in presence of both triclosan and NADP+
-
F204S
-
no shift in melting temperature in presence of both triclosan and NADP+
-
I193S
-
no shift in melting temperature in presence of both triclosan and NADP+
-
Y147H
-
mutant is resistant to antibiotic AFN-1252 and to triclosan, about 5% of wild-type catalytic activity. A strain expressing Y147H has a pronounced growth defect that is rescued by exogenous fatty acid supplementation
-
additional information
replacement of amino acids 55-66 by CurF enoyl reductase cyclopropanase loop leads to gain-of-function activity as a cyclopropanase. With substrate 3-chloromethyl-crotonyl-[acyl-carrier-protein], the formation of both the reduced product and also the cyclopropanated productis detected at levels of about one-quarter of the total product