1.3.1.116: 7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase

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For detailed information about 7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase, go to the full flat file.

Reaction

Synonyms

7beta-hydroxy-3-oxo-DELTA4-cholenoic acid oxidoreductase, BaiH

ECTree

1 Oxidoreductases

1.3 Acting on the CH-CH group of donors

1.3.1 With NAD⁺ or NADP⁺ as acceptor

1.3.1.116 7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase

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Results	in table
1	AA Sequence
4	Cloned(Commentary)
10	Cofactor
3	Expression
6	General Information
26	Inhibitors
4	Metals/Ions
6	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
21	Organism
2	Pathway
3	pH Optimum
1	pI Value
4	Purification (Commentary)
1	Reaction
9	Reference
3	Specific Activity [micromol/min/mg]
15	Substrates and Products (Substrate)
16	Subunits
12	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]

Cloned

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Cloned on EC 1.3.1.116 - 7beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase

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[Clostridium] scindens

P32370

745196

expression in Escherichia coli

2 entries

subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than does Eubacterium sp. VPI 12708 cells induced with cholic acid

[Clostridium] scindens

P32370

744377