1.3.1.13: prephenate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about prephenate dehydrogenase (NADP+), go to the full flat file.

Reaction

Synonyms

11415867, CM-PD, MtPDH1, PD-CM-PDT, PDH1, PRDH, prephenate (nicotinamide adenine dinucleotide phosphate) dehydrogenase, prephenate dehydrogenase, prephenate-pretyrosine dehydrogenase, tyrA, TyrAp, XD72_2094

ECTree

     1 Oxidoreductases

         1.3 Acting on the CH-CH group of donors

             1.3.1 With NAD⁺ or NADP⁺ as acceptor

                1.3.1.13 prephenate dehydrogenase (NADP+)

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Results	in table
900	AA Sequence
1	CAS Registry Number
4	Cloned(Commentary)
20	Cofactor
3	General Information
12	Inhibitors
18	kcat/KM [mM/s]
2	Ki Value [mM]
35	KM Value [mM]
2	Localization
5	Molecular Weight [Da]
26	Natural Substrates/ Products (Substrates)
40	Organism
3	Pathway
8	PDB ID
3	pH Optimum
1	pH Range
1	pH Stability
21	Protein Variants
6	Purification (Commentary)
1	Reaction
4	Reaction Type
39	Reference
11	Source Tissue
1	Specific Activity [micromol/min/mg]
30	Substrates and Products (Substrate)
3	Subunits
19	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
4	Temperature Stability [°C]
18	Turnover Number [1/s]

Engineering

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Engineering on EC 1.3.1.13 - prephenate dehydrogenase (NADP+)

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

H124A

Glycine max

-

inactive

762908

H124N

Glycine max

-

substantial decrease in catalytic efficiency

762908

H124Q

Glycine max

-

inactive

762908

H188A

Glycine max

-

inactive

762908

H188N

Glycine max

-

inactive

762908

H188Q

Glycine max

-

inactive

762908

N222A

Glycine max

-

complete loss of activity

762908

N222Q

Glycine max

-

30fold decrease in kcat value and a modest change in Km value

762908

Q130A

Glycine max

-

300fold lower turnover rate

762908

Q130D

Glycine max

-

4fold reduction in kcat values and less than twofold increase in Km value

762908

Q130E

Glycine max

-

4fold reduction in kcat values and less than twofold increase in Km value

762908

Q130S

Glycine max

-

35fold lower turnover rate

762908

Q184A

Glycine max

-

320fold lower kcat value, with a Km value comparable to that of the wild-type

762908

Q184D

Glycine max

-

loss of detectable PDH activity

762908

Q184E

Glycine max

-

loss of detectable PDH activity

762908

S101A

Glycine max

-

mutantion primarily affectes the Km value for prephenate with a 20fold increase

762908

T131A

Glycine max

-

134fold decrease in kcat value

762908

T131S

Glycine max

-

272fold decrease in kcat value

762908

T206A

Glycine max

-

loss of detectable activity

762908

T206S

Glycine max

-

tremendous loss of activity

762908

Q227E

Methanothrix harundinacea

A0A101IGG2

mutation introduces arogenate dehydrogenase activity

763088