1.3.1.14: dihydroorotate dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about dihydroorotate dehydrogenase (NAD+), go to the full flat file.

Reaction

Synonyms

b-type dihydroorotate dehydrogenase, DHO dehydrogenase, DHODase, DHOdehase A, dihydro-orotic dehydrogenase, dihydroorotate dehydrogenase, dihydrorotate dehydrogenase B, L-5,6-dihydro-orotate:NAD oxidoreductase, More, orotate reductase, reductase, orotate

ECTree

     1 Oxidoreductases

         1.3 Acting on the CH-CH group of donors

             1.3.1 With NAD⁺ or NADP⁺ as acceptor

                1.3.1.14 dihydroorotate dehydrogenase (NAD+)

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Results	in table
14	Activating Compound
824	AA Sequence
7	Application
1	CAS Registry Number
6	Cloned(Commentary)
67	Cofactor
2	Crystallization (Commentary)
1	General Stability
32	Inhibitors
11	Ki Value [mM]
19	KM Value [mM]
8	Localization
19	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
24	Organism
5	Pathway
4	PDB ID
6	pH Optimum
4	pH Range
2	pH Stability
9	Protein Variants
9	Purification (Commentary)
9	Reaction
4	Reaction Type
22	Reference
2	Renatured (Commentary)
9	Specific Activity [micromol/min/mg]
7	Storage Stability
54	Substrates and Products (Substrate)
14	Subunits
14	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
15	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.3.1.14 - dihydroorotate dehydrogenase (NAD+)

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-18°C, prepared enzyme, that is a yellow powder, several weeks, stable

Faecalicatena orotica

-

390563

-20°C, 0.27 M sodium phosphate, pH 6.3, over 6 months, less than 15% loss of activity

Faecalicatena orotica

-

390568

4°C, crystals, over a month, little or no loss of activity

Faecalicatena orotica

-

390566

4°C, holoenzyme, stable for many days

Bacillus subtilis

-

390928

above 20°C, 0.2 M phosphate buffer, fast loss of activity

Faecalicatena orotica

-

390564

on ice at room temperature, PyrDI, gradually loses activity over a period of hours

Bacillus subtilis

-

390928

room temperature, holoenzyme, stable for many hours

Bacillus subtilis

-

390928