1.3.1.19: cis-1,2-dihydrobenzene-1,2-diol dehydrogenase
This is an abbreviated version!
For detailed information about cis-1,2-dihydrobenzene-1,2-diol dehydrogenase, go to the full flat file.
Word Map on EC 1.3.1.19
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1.3.1.19
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putida
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catechols
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dioxygenase
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dehydrogenases
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plasmid-borne
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hydrocarbons
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cis-dihydrodiols
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2,3-dioxygenase
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nad+-dependent
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nadp+
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analysis
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synthesis
- 1.3.1.19
- putida
- catechols
- dioxygenase
- dehydrogenases
-
plasmid-borne
- hydrocarbons
- cis-dihydrodiols
-
2,3-dioxygenase
-
nad+-dependent
- nadp+
- analysis
- synthesis
Reaction
Synonyms
benzene dihydrodiol dehydrogenase, cis-1,2-dihydrocyclohexa-3,5-diene oxidoreductase, cis-benzene dihydrodiol dehydrogenase, cis-benzene glycol dehydrogenase, naphthalene cis-1,2-dihydrodiol dehydrogenase, NDDH
ECTree
1.3.1.19 cis-1,2-dihydrobenzene-1,2-diol dehydrogenaseAdvanced search results
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Application on EC 1.3.1.19 - cis-1,2-dihydrobenzene-1,2-diol dehydrogenase
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APPLICATION 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
analysis
synthesis
analysis
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Escherichia coli, which carry genes coding for benzene dioxygenase and benzene dihydrodiol dehydrogenase, can be used to monitor benzene pollution in environmental airsamples collected from an oil refinery. The procedures involving whole-cell bioassays determine the concentration of benzene through benzene dioxygenase activity, which allows for direct correlation of oxygen consumption, and through the benzene dihydrodiol dehydrogenase that causes catechol accumulation and restores NADH necessary for the activity of the first enzyme. The assay is sensitive enough to detect the benzene vapor at a concentration level of 0.01 mM in about 30 min. The assay is applicalble to on-line monitoring of benzene concentration, no particular treatment of environmental samples is required
analysis
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Escherichia coli, which carry genes coding for benzene dioxygenase and benzene dihydrodiol dehydrogenase, can be used to monitor benzene pollution in environmental airsamples collected from an oil refinery. The procedures involving whole-cell bioassays determine the concentration of benzene through benzene dioxygenase activity, which allows for direct correlation of oxygen consumption, and through the benzene dihydrodiol dehydrogenase that causes catechol accumulation and restores NADH necessary for the activity of the first enzyme. The assay is sensitive enough to detect the benzene vapor at a concentration level of 0.01 mM in about 30 min. The assay is applicalble to on-line monitoring of benzene concentration, no particular treatment of environmental samples is required
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synthesis
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preparation of two recombinant strains each containing two enzymatic activities mutually expressed through regulated systems for production of functionalized epoxides in one-pot reactions. The gene coding for styrene monooxygenase from Pseudomonas fluorescens ST and the gene coding for naphthalene dihydrodiol dehydrogenase from Pseudomonas fluorescens N3 are expressed either in Pseudomonas putida PaW340 or in Escherichia coli. The combination of the enzymes allows to obtain the transformation of allylic primary alcohols into alpha,beta-epoxy acids
synthesis
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preparation of two recombinant strains each containing two enzymatic activities mutually expressed through regulated systems for production of functionalized epoxides in one-pot reactions. The gene coding for styrene monooxygenase from Pseudomonas fluorescens ST and the gene coding for naphthalene dihydrodiol dehydrogenase from Pseudomonas fluorescens N3 are expressed either in Pseudomonas putida PaW340 or in Escherichia coli. The combination of the enzymes allows to obtain the transformation of allylic primary alcohols into alpha,beta-epoxy acids
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