1.3.1.3: DELTA4-3-oxosteroid 5beta-reductase

This is an abbreviated version!
For detailed information about DELTA4-3-oxosteroid 5beta-reductase, go to the full flat file.

Word Map on EC 1.3.1.3

Reaction

17,21-dihydroxy-5beta-pregnane-3,11,20-trione
+
NADP+
=
cortisone
+
NADPH
+
H+

Synonyms

3-oxo-DELTA4-steroid 5beta-reductase, 4,5beta-dihydrocortisone:NADP+ DELTA4-oxidoreductase, 5-beta-reductase, 5beta-POR, 5beta-red, AKR1D1, aldo-keto reductase, aldo-keto reductase 1D1, aldo–keto reductase 1D1, androstenedione 5beta-reductase, cortisone 5beta-reductase, cortisone beta-reductase, DELTA 4-5beta-reductase, DELTA4-3-ketosteroid 5beta-reductase, DELTA4-3-oxosteroid 5beta-reductase, DELTA4-hydrogenase, EC 1.3.1.3, h5beta-red, h5beta-reductase, More, P5betaR, progesterone 5beta-reductase, reductase, cholestenone 5beta.-, reductase, cortisone DELTA4-5beta-, steroid 5beta-reductase, steroid 5beta.-reductase, testosterone 5-beta-reductase, testosterone 5beta-reductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.3 DELTA4-3-oxosteroid 5beta-reductase

Cloned

Cloned on EC 1.3.1.3 - DELTA4-3-oxosteroid 5beta-reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutant enzymes in Escherichia coli strain M15
expressed in Escherichia coli
-
expressed in Escherichia coli strain C41(DE3)
-
expressed in Escherichia coli strain M15
-
expression in Escherichia coli and HEK-293 cell; recombinant expression of AKR1D1 enzyme mutants in HEK-293 cells
-
expression in human HEK-293 cells; pseudogene found
-
expression of GST-tagged enzyme in Escherichia coli
-
full-length P5betaR cDNA overexpressed in Escherichia coli as a fusion protein with MBP or His-tag
-
gene SRD5B1, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes
overproduced by the pQE expression system and transformed into Escherichia coli strain B834(DE3)
-
recombinant expression of AKR1D1 enzyme mutants in HEK-293 cells only at low levels possible and with low activities
-
Sph1/Sal1 5beta-POR fragment cloned into the pQE vector and transformed into Escherichia coli strain M15(pREP4)
-
SPHI /SALI IC5beta-POR fragment cloned into the pQE vector system and transformed into Escherichai coli strain M15(pREP4)