1.3.1.42: 12-oxophytodienoate reductase

This is an abbreviated version!
For detailed information about 12-oxophytodienoate reductase, go to the full flat file.

Word Map on EC 1.3.1.42

Reaction

8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate
+
NADP+
=
(15Z)-12-oxophyto-10,15-dienoate
+
NADPH
+
H+

Synonyms

12-oxo phytodienoic acid reductase, 12-oxo-phytodienoate acid reductase, 12-oxo-phytodienoic acid reductase, 12-oxophytodienoate reductase, 12-oxophytodienoate reductase 3, 12-oxophytodienoate reductase isoform 3, 12-oxophytodienoate-10,11-reductase, 12-oxophytodienoate10,11-reductase, CsOPR3, cyclopentenone reductase, HvOPR1, More, morphine reductase, old yellow enzyme, OPDA reductase, OPDA reductase I, OPR, OPR-1, OPR-3, OPR1, OPR2, OPR3, OPR3 oxidoreductase, OPR4, OPR5, OPR6, OPR7, OPR8, OPRI, OPRII, OsOPR01-1, OsOPR02-1, OsOPR04-1, OsOPR06-1, OsOPR08-1, OsOPR10, OsOPR11, OsOPR6, OsOPR7, OsOPR8, oxophytodienoate reductase, oxophytodienoic acid reductase, OYE, phytodienoic acid reductase, S64, TaOPR1

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.42 12-oxophytodienoate reductase

Cloned

Cloned on EC 1.3.1.42 - 12-oxophytodienoate reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
c-DNA library, gene OPR3 identified
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cDNA library construction, protein S64, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as His-tagged protein
cloned and expressed in Escherichia coli
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cloning and expression of a gene encoding old yellow enzyme
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cloning, and DNA sequence determination and analysis, expression of gene At2g06050, encoding isozyme OPR3, in Escherichia coli as N-terminally fusion protein fused to S-tag-His6-maltose binding protein
-
construction of a cDNA library, phylogenetic analysis, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3)
construction of a genomic DNA library, identification and DNA sequence comparison of 6 genes of OPR belonging to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis, DNA sequence and gene structure determination and analysis, expression of isozymes in Escherichia coli strain BL21(DE3); construction of a genomic DNA library, identification of 6 genes of OPR belonging to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis, DNA sequence and gene structure determination and analysis, expression of isozymes in Escherichia coli strain BL21(DE3)
enzyme cloned from its cDNA, open reading frame encodes a 372 amino acids polypeptide, functionally expressed in Escherichia coli
expressed in Arabidopsis thaliana through transformation with Agrobacterium tumefaciens strain EHA105
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expressed in Arabidopsis thaliana through transformation with Agrobacterium tumefaciens strain GV3101
-
expressed in Escherichia coli
expression of His-tagged isozyme OPR1 in Escherichia coli, transient expression of isozyme OPR1 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study; expression of His-tagged isozyme OPR2 in Escherichia coli; expression of His-tagged isozyme OPR3 in Escherichia coli, transient expression of isozyme OPR3 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study
expression of the 3 isozymes OPR1-3 as N-terminally GST- or His-tagged enzymes in Escherichia coli
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gene CsOPR3, a putative OPR gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis
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gene TaOPR1, TaOPR1 maps to the short arm of the second chromosome of the B genome (2BS), DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression in Arabidopsis thaliana ecotype Col-0. The repression of AtOPR1 by the TaOPR1 transgene suggests a negative feedback regulatory effect, perhaps based on functional redundancy between TaOPR1 and AtOPR1
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generating of a jasmonic acid-deficient rice line with suppressed expression of the gene encoding OPR3 with resistance to Magnaporthe grisea by introduction into rice plants by Agrobacterium-mediated transformation, OPR-interfering RNA plants show normal resistance to both an incompatible and a compatible race of Magnaporthe grisea; generating of a jasmonic acid-deficient rice line with suppressed expression of the genes encoding OPR1 with resistance to Magnaporthe grisea by introduction into rice plants by Agrobacterium-mediated transformation, OPR-interfering RNA plants show normal resistance to both an incompatible and a compatible race of Magnaporthe grisea
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview
OPR1 and OPR2, two closely related genes encodes 12-oxophytodienoic acid-10,11-reductases
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OPR3 expressed in functional form in Escherichia coli, cloning of OPR2 cDNA by reverse transcriptase-polymerase chain reaction, OPR2 expressed in insect cell cultures of Spodoptera frugiperda with transgenic Autographa californica baculoviruses and overexpressed in transgenic Arabidopsis thaliana
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OPR3 mutant enzymes are expressed in Escherichia coli
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recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR1; recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR2; recombinant expression of OPR3 in Escherichia coli
subcloning of the OPR genes from a lambdaGEM11 genomic library
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