1.3.1.42: 12-oxophytodienoate reductase

This is an abbreviated version!
For detailed information about 12-oxophytodienoate reductase, go to the full flat file.

Word Map on EC 1.3.1.42

Reaction

8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate
+
NADP+
=
(15Z)-12-oxophyto-10,15-dienoate
+
NADPH
+
H+

Synonyms

12-oxo phytodienoic acid reductase, 12-oxo-phytodienoate acid reductase, 12-oxo-phytodienoic acid reductase, 12-oxophytodienoate reductase, 12-oxophytodienoate reductase 3, 12-oxophytodienoate reductase isoform 3, 12-oxophytodienoate-10,11-reductase, 12-oxophytodienoate10,11-reductase, CsOPR3, cyclopentenone reductase, HvOPR1, More, morphine reductase, old yellow enzyme, OPDA reductase, OPDA reductase I, OPR, OPR-1, OPR-3, OPR1, OPR2, OPR3, OPR3 oxidoreductase, OPR4, OPR5, OPR6, OPR7, OPR8, OPRI, OPRII, OsOPR01-1, OsOPR02-1, OsOPR04-1, OsOPR06-1, OsOPR08-1, OsOPR10, OsOPR11, OsOPR6, OsOPR7, OsOPR8, oxophytodienoate reductase, oxophytodienoic acid reductase, OYE, phytodienoic acid reductase, S64, TaOPR1

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.42 12-oxophytodienoate reductase

Purification

Purification on EC 1.3.1.42 - 12-oxophytodienoate reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by affinity chromatography; by affinity chromatography; by affinity chromatography
OPR1, OPR2 and OPR3
-
purified in enzymatically active form
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant His-tagged isozyme OPR1 from Escherichia coli by nickel affinity chromatography; recombinant His-tagged isozyme OPR2 from Escherichia coli by nickel affinity chromatography; recombinant His-tagged isozyme OPR3 from Escherichia coli by nickel affinity chromatography
recombinant isozyme from Escherichia coli
recombinant isozyme OPR3 from Escherichia coli by nickel-imino-diacetic acid affinity chromatography
-
recombinant protein
-
recombinant tagged isozymes OPR1-3 from Escherichia coli by affinity chromatography
-