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1.3.1.57: phloroglucinol reductase

This is an abbreviated version!
For detailed information about phloroglucinol reductase, go to the full flat file.

Word Map on EC 1.3.1.57

Reaction

dihydrophloroglucinol
+
NADP+
=
phloroglucinol
+
NADPH
+
H+

Synonyms

PGR, PGRcl, PGRde, PGReu

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.57 phloroglucinol reductase

Purification

Purification on EC 1.3.1.57 - phloroglucinol reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
prepared of a cell extract by French press, DEAE-cellulose column, phenyl-Sepharose column, ultrafiltration, red agarose column, anion exchange column, purified 90.4fold with a yield of 29%
-
protamine sulfate precipitation, ammonium sulfate precipitation, centrifugation steps, dialysis, gel filtration
-
recombinant His-tagged enzyme from Escherichia coli train BL21(DE3) by nickel affinity chromatography