1.3.1.6: fumarate reductase (NADH)

This is an abbreviated version!
For detailed information about fumarate reductase (NADH), go to the full flat file.

Word Map on EC 1.3.1.6

Reaction

succinate
+
NAD+
=
fumarate
+
NADH
+
H+

Synonyms

FRD, FRdABCD, FRDg, FRDm1, FRDm2, FRDS, Frds1p, fumarate reductase, mitochondrial rhodoquinol-fumarate reductase, NADH-dependent fumarate reductase, NADH-FR, NADH-FRD, NADH-fumarate reductase, NFRD, QFR

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.6 fumarate reductase (NADH)

Engineering

Engineering on EC 1.3.1.6 - fumarate reductase (NADH)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H505A
-
site-directed mutagenesis, altered binding structure of the sodium ion, crystal structure analysis, at pH 8.5 the activity of the mutant enzyme is similar to the wild-type enzyme, at pH 6.0 the ctivity is 20fold reduced
H505Y
-
site-directed mutagenesis, altered binding structure of the sodium ion, crystal structure analysis, at pH 8.5 the activity of the mutant enzyme is similar to the wild-type enzyme
H61A
-
site-directed mutagenesis, 5-10fold reduced kcat compared to the wild-type enzyme, elevated pH optimum, electrochemical analysis, crystal structure analysis, altered ligand binding of hemes and heme iron, 80% of wild-type activity can be recovered by addition of exogenous imidazole, overview
H61M
-
site-directed mutagenesis, 5-10fold reduced kcat compared to the wild-type enzyme, elevated pH optimum, electrochemical analysis, crystal structure analysis, altered ligand binding of hemes and heme iron, 80% of wild-type activity can be recovered by addition of exogenous imidazole, overview
additional information