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1.3.1.72: DELTA24-sterol reductase

This is an abbreviated version!
For detailed information about DELTA24-sterol reductase, go to the full flat file.

Word Map on EC 1.3.1.72

Reaction

5alpha-Cholest-7-en-3beta-ol
+
NADP+
=
5alpha-cholesta-7,24-dien-3beta-ol
+
NADPH
+
H+

Synonyms

24,25-reductase, 24,25-sterol reductase, 24-dehydrocholesterol reductase, 24-reductase, 3 beta-hydroxysteroid-DELTA 24 reductase, 3-beta-hydroxysterol-DELTA24-reductase, 3beta -hydroxysterol DELTA24-reductase, 3beta-hydroxysteroid-DELTA24 reductase, 3beta-hydroxysterol DELTA24-reductase, 3beta-hydroxysterol-delta24 reductase, ArDWF1, C-24 reductase, dehydrocholesterol reductase, DELTA24 reductase, DELTA24-dehydrocholesterol reductase, DELTA24-reductase, DELTA24-sterol-DELTA24-reductase, desmosterol reductase, DHCR24, DHCR24-1, DHCR24-2, DIM, DIMINUTO/DWARF1, Dimunito/Dwarf1, DWARF1, DWF1, hydroxy steroid DELTA24-reductase, lanosterol 24-reductase, lanosterol DELTA24-reductase, lanosterol reductase, More, OsDWF1, reductase, hydroxy steroid DELTA24-, seladin-1, sterol 24,25-reductase, sterol 24-reductase, sterol 24DELTA-reductase, sterol C-24 reductase, sterol DELTA24 reductase, sterol DELTA24-reductase, sterol-DELTA24-reductase, ZmDWF1

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.72 DELTA24-sterol reductase

Cloned

Cloned on EC 1.3.1.72 - DELTA24-sterol reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of a recombinant adenovirus driving DHCR24 expression vector specific for neurons with neuron-specific promoter, synapsin-1 (SYN1), cloning of a SYN1 promoter DNA fragment of human enzyme DHCR24, and transfection of recombinant Ad-hSYN1-DHCR24 into HEK AD-293, N2A (mouse neuroblastoma), and MIN6 (mouse pancreatic carcinoma) cells. DHCR24 is specially expressed in HEK AD-293 and N2A cells, but not in MIN6 cells. Adenovirus transfection inhibits apoptosis through scavenging excess reactive oxygen species, and recombinant DHCR24 adenoviruse induces neuron-specific DHCR24 expression
construction of a recombinant adenovirus driving DHCR24 expression vector specific for neurons with neuron-specific promoter, synapsin-1 (SYN1), cloning of a SYN1 promoter DNA fragment of rat enzyme DHCR24, and transfection of recombinant Ad-rSYN1-DHCR24 into HEK AD-293, N2A (mouse neuroblastoma), and MIN6 (mouse pancreatic carcinoma) cells. DHCR24 is specially expressed in HEK AD-293 and N2A cells, but not in MIN6 cells. Adenovirus transfection inhibits apoptosis through scavenging excess reactive oxygen species, and recombinant DHCR24 adenoviruse induces neuron-specific DHCR24 expression
DWF1, phylogenetic tree
-
ectopic expression of wild-type and mutant human DHCR24s in CHO-7 cells that are deficient for DHCR24 through specific siRNA knockout, quantitative real-time RT-PCR enzyme expression analysis
expressed as a EGFP-fusion protein in murine neuroblastoma cell line N2A
-
expressed in CHO cells
-
expressed in HEK-293 cells
-
expressed in Mus musculus embryonic fibroblasts and PC-12 cells infected with adenovirus
-
gene ArDWF1, cloning from hairy root expression sequence tag library, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, functional recombinant expression in Saccharomyces cerevisiae, ArDWF1-expressing yeast T21 cells produce 24-methylcholesterol in vivo
gene DHCR24, DNA and amino acid sequence determination and analysis of wild-type and natural desmosterolysis mutant genes, chromosomal mapping to 1p31.1-p33, functional expression of wild-type and mutant enzymes in Saccharomyces cerevisiae
-
gene OsDWF1, sequence comparison and phylogenetic tree, functional recombinant expression in Saccharomyces cerevisiae, OsDWF1-expressing yeast T21 cells produce 24-methylcholesterol in vivo
-
gene ZmDWF1, single copy gene, DNA and amino acid sequence determination and analysis, cloning of a plant expression vector for construction of transgenic plants
generation of plasmids pcDNA5-DHCR7-myc/FRT and pcDNA5-DHCR24-V5/FRT, and of DHCR24 mutant plasmid pcDNA5-DHCR24 Y471S-V5/FRT, the latter is stably recombinantly expressed in CHO-7 cells, stable overexpression of inactive DHCR24 mutant Y471S in CHO-7 cells, quantitative real-time PCR expression analysis
HEK-293T cells
-
overexpressed in ATDC-5 cells
-
quantitative real-time PCR DHCR24 expression analysis
-
recombinant expression of C-terminally YFP-tagged wild-type enzyme in Arabidopsis thaliana mutant lines deficient in the enzyme in the endoplasmic reticulum and plasma membrane
recombinant expression of truncated DHCR24 (DELTA23 DHCR24) with a V5 epitope tag at either the N-terminus (V5-DHCR24) or C-terminus (DHCR24-V5) in CHO-7 cells, and the cellular localization of DELTA23 DHCR24 is primarily to the membrane fraction like the wild-type
RzM6-0d cells are transduced with DHCR24 lentivirus. Expression of DHCR24 from pGEM-T easy vector via in vitro translation using reticulocyte lysate. Expression of HA- or FLAG-tagged DHCR24 in RzM6 or HepG2 cells. Expression of GFP- and FLAG-tagged DHCR24 in 293FT cells
-
the DHCR24 gene locus is 1p32.3, spans about 46.4 kb, and comprises eight introns and nine exons, promoter map of human DHCR24, phylogenetic tree