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1.3.1.84: acrylyl-CoA reductase (NADPH)

This is an abbreviated version!
For detailed information about acrylyl-CoA reductase (NADPH), go to the full flat file.

Word Map on EC 1.3.1.84

Reaction

propanoyl-CoA
+
NADP+
=
acryloyl-CoA
 +
NADPH
 +
H+

Synonyms

Acr, acryloyl-CoA reductase, acryloyl-CoA reductase (NADPH), acryloyl-coenzyme A reductase, acrylyl-CoA reductase, acrylyl-coenzyme A reductase, AcuI, Msed_1426, NADPH-dependent acryloyl-CoA reductase activity, NADPH-dependent acrylyl-CoA reductase, RSP_1434, SPO1914, SPO_1914, yhdH

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.84 acrylyl-CoA reductase (NADPH)

Cloned

Cloned on EC 1.3.1.84 - acrylyl-CoA reductase (NADPH)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta2 (DE3) cells
gene Msed_1426, cloned from genomic DNA, recombinant co-expression of His-tagged enzyme with enzyme succinic semialdehyde reductase (NADPH), SSR, gene Msed_1424 from Metallosphaera sedula, in Escherichia coli strain Rosetta 2(DE3). Construction of a yeast two-hybrid assay for protein interaction analysis of ACR and SSR. ACR cannot be expressed in soluble, active form in Escherichia coli
gene RSP_1434, recombinant expression of N-terminally His10-tagged enzyme in Escherichia coli strain Rosetta2 (DE3)
gene SPO1914, the gene is adjacent to and predicted to be within the same transcriptional unit as dmdA, which encodes the enzyme for the first step of the demethylation pathway
gene SPO_1914, recombinant expression of N-terminally His10-tagged enzyme in Escherichia coli strain Rosetta2 (DE3), recombinant expression in Rhodobacter sphaeroides mutant DELTAacuI::kan complements 3-hydroxypropionate-dependent growth of the mutant strain
gene yhdH, cloning and expression of N-terminally His10-tagged enzyme in Escherichia coli strain Rosetta2 (DE3), recombinant expression in Rhodobacter sphaeroides mutant DELTAacuI::kan complements 3-hydroxypropionate-dependent growth of the mutant strain