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1.3.1.97: botryococcene synthase

This is an abbreviated version!
For detailed information about botryococcene synthase, go to the full flat file.

Word Map on EC 1.3.1.97

Reaction

C30 botryococcene
+
NADP+
 +
diphosphate
=
presqualene diphosphate
 +
NADPH
 +
H+

Synonyms

botryococcene synthase, BSS, squalene synthase-like 3, squalene synthase-like protein 3, SSL-3

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.97 botryococcene synthase

Cloned

Cloned on EC 1.3.1.97 - botryococcene synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Chlamydomonas reinhardtii chloroplasts
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Saccharomyces cerevisiae strain TN7
gene SSL-3, recombinant expression of the enzyme in Nicotiana tabacum, ene constructs consisted of a peptide fusion of SSL-1 and SSL-3 connected by a triplet repeat peptide linker of Gly-Gly-Ser-Gly, with or without appending the C-terminal end (71 amino acids) of the Botryococcus braunii squalene synthase onto the C-terminus of SSL-3 and the FPS gene. The chimeric SSL1-3 genes and FPS gene are inserted downstream of the strong constitutive promoters Pcv and Pca, respectively. For trichome-specific expression of triterpene biosynthesis, the trichome-specific promoters Pcbt and Pcyp16 are fused to 5' end of botryococcene synthase gene, presqualene diphosphate synthase gene, and the FPS gene, respectively
gene SSL-3, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)