1.3.1.98: UDP-N-acetylmuramate dehydrogenase

This is an abbreviated version!
For detailed information about UDP-N-acetylmuramate dehydrogenase, go to the full flat file.

Word Map on EC 1.3.1.98

Reaction

UDP-N-acetyl-alpha-D-muramate
+
NADP+
=
UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
+
NADPH
+
H+

Synonyms

alr5066, EC 1.1.1.158, More, MurB, MurBAb, PA2977, PaMurB, reductase, uridine diphosphoacetylpyruvoylglucosamine, type I UNAGEP reductase, UDP-GlcNAc-enoylpyruvate reductase, UDP-N-acetylenolpyruvoylglucosamine reductase, UDP-N-acetylenolpyruvyl glucosamine reductase, UDP-N-acetylenolpyruvylglucosamine reductase, UDP-N-acetylglucosamine-enoylpyruvate reductase, UDP-N-acetylmuramate dehydrogenase, UNAGEP reductase, uridine diphospho-N-acetylglucosamine-enolpyruvate reductase, uridine-5'-diphospho-N-acetyl-2-amino-2-deoxy-3-O-lactylglucose:NADP-oxidoreductase, VspiD_010100018130

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.98 UDP-N-acetylmuramate dehydrogenase

Engineering

Engineering on EC 1.3.1.98 - UDP-N-acetylmuramate dehydrogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S229A
-
by site-directed mutagenesis
S226A
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, no complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
R176A
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, no complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
R213A
site-directed mutagenesis, nearly inactive mutant, no complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
R230A
site-directed mutagenesis, functional complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
N71A
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, no complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
S226A
-
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, no complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
S243F
strong decrease in enzyme activity, thinner cell walls than wild type strain
S70A
site-directed mutagenesis, functional complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
Y175F
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, poor functional complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
N71A
-
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, no complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
E275K
strong decrease in enzyme activity, thinner cell walls than wild type strain
H259A
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, no complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
G78D
strong decrease in enzyme activity, thinner cell walls than wild type strain
G69A
site-directed mutagenesis, functional complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
G67A
site-directed mutagenesis, functional complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
G251D
strong decrease in enzyme activity, thinner cell walls than wild type strain
E296A
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, no complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901
E275K
-
strong decrease in enzyme activity, thinner cell walls than wild type strain
additional information