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1.3.3.12: L-galactonolactone oxidase

This is an abbreviated version!
For detailed information about L-galactonolactone oxidase, go to the full flat file.

Word Map on EC 1.3.3.12

Reaction

L-galactono-1,4-lactone
+
O2
=
L-ascorbate
+
H2O2

Synonyms

EC 1.1.3.24, GAL, galactone-gamma-lactone oxidase, galactonolactone oxidase, galactonolactone oxidoreductase, GL oxidase, L-galactono-1,4-lactone oxidase, L-galactono-gamma-lactone oxidase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.12 L-galactonolactone oxidase

Engineering

Engineering on EC 1.3.3.12 - L-galactonolactone oxidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A113G
-
site-directed mutagenesis, in the reaction with 1,4-benzoquinone as electron acceptor, the activity of the dimeric formof A113G is about 1.5fold higher compared to the monomer, the specific activity of the A113G variant in the reaction with molecular oxygen is about 2.5-3times lower than with 1,4-benzoquinone
H447G
-
the mutant is inactive and shows undetectable FMN binding
K450G
-
the substitution results in an enzyme that retains an ability to bind FMN (around 50%of the wild type enzyme), but which exhibits no activity
K55G
-
the mutation has only a minor inhibitory effect on both FMN binding and biochemical activity
K55H
-
the mutation causes dramatically reduced FMN binding and enzyme activity (above 90%)
K55L
-
the mutant is inactive and shows undetectable FMN binding
W448G
-
the mutant is inactive and shows undetectable FMN binding
A113G
-
site-directed mutagenesis, in the reaction with 1,4-benzoquinone as electron acceptor, the activity of the dimeric formof A113G is about 1.5fold higher compared to the monomer, the specific activity of the A113G variant in the reaction with molecular oxygen is about 2.5-3times lower than with 1,4-benzoquinone
-