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1.3.3.4: protoporphyrinogen oxidase

This is an abbreviated version!
For detailed information about protoporphyrinogen oxidase, go to the full flat file.

Word Map on EC 1.3.3.4

Reaction

protoporphyrinogen IX
+ 3 O2 =
protoporphyrin IX
 + 3 H2O2

Synonyms

HemG, HemG-type PPO, HemG-type protoporphyrinogen IX oxidase, hemY, hPPO, H_N10, H_N40, H_N90, LMJF_06_1280, mtPPO, MxPPOX, MxProtox, PPO, PPO1, ppo1-1, PPO2, PPOX, PPOX I, PPX1, PPX2, protein YfeX, protogen oxidase, protoporphyrinogen IX oxidase, protoporphyrinogen IX oxidase 1, protoporphyrinogen oxidase, protoporphyrinogen oxidase IX, protoporphyrinogenase, protox, Protox enzyme, R-PPO, Rs-slr1790 protein, S-PPO, Salk_143057, YfeX

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.4 protoporphyrinogen oxidase

Cloned

Cloned on EC 1.3.3.4 - protoporphyrinogen oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a modified, low G+C-content MxProtox gene construct pGA1611:synthetic MxProtox is transformed into Oryza sativa using Agrobacterium tumefaciens-mediated transformation altering the sensitivity of rice plants to herbicide oxyfluorfen, quantitative reverse transcriptase PCR expression analysis. Oxyfluorfen induces changes in the expression of the modified MxProtox gene, constitutive OsProtox gene, and other key genes encoding the porphyrin pathway, in leaves of the Oryza sativa wild-type and transgenic lines, overview
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cloned into plasmid pET28(b), expression in Escherichia coli BL21(DE3), subcloned into pGA1611 under the control of constitutive maize ubiquitin promoter and expressed in transgenic rice plant lines S11 and S19
AY916795
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli and as GFP-fusion proteins in COS-1 cells
enzyme is nuclearly encoded, DNA sequence determination and analysis of native, recombinant, and processed enzyme, expression in Escherichia coli
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expressed in Escherichia coli BL21(DE3)pLysS cells
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expressed in Escherichia coli HemG-knock-out strain as well as maize, soybean cotton, and canola
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expressed in Escherichia coli JM109 cells
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expressed in Escherichia coli strain BL21(DE3)RIL
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expressed in Escherichia coli strain BT3
expressed in Oryza sativa chloroplasts
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expressed in Oryza sativa cultivar Dongjin
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expressed in Oryza sativa line M4 chloroplasts and mitochondria
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expression in Escherichia coli BL21(DE3)RIL containing the original or mutated pET32a-PPO2 plasmids
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expression in transgenic rice plant lines M4 and and M7
expression of His-tagged enzyme in Escherichia coli
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expression of the enzyme in transgenic rice plants using Agrobacterium tumefaciens transformation system
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expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10b
Thermosynechococcus vestitus
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expression of the His-tagged enzyme in Escherichia coli strain JM109
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expression of the thioredoxin- and His-tagged enzyme in Escherichia coli strain B834(DE3), selenomethionine labeling in the auxothrophic strain
expression of wild-type and mutant enzymes in Escherichia coli enzyme-deficient strain SAS38X which is not able to grow without exogenously added heme, complementation study, comparison of missense mutations in the human PPOX gene with the equivalent positions of other species' genes
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expression of wild-type and mutant enzymes in Escherichia coli strain JM109
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expression of wild-type and mutant enzymes in Escherichia coli strain XL-1 Blue in inclusion bodies
expression of wild-type and mutant V311M as His-tagged enzymes in Escherichia coli strain BL21(DE3)
full-length cDNA encoding Protox fused to a plastidal transit sequence at its N-terminus and subcloned into pGA1611 under the control of the constitutive maize ubiquitin promoter, with the hygromycin phosphotransferase gene as a selectable marker, overexpression in transgenic rice plants
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gene hemeY, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain JM109, growth at 30°C (rather than at 37°C) improves expression, and yield is increased by doubling the number of cells inoculated in 1 l growth media
gene hemG, DNA and amino acid sequence determination and analysis
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gene hemY or slr1790, DNA and amino acid sequence determination and analysis
gene hemY, recombinant expression in Oryza sativa cv. Dongjin constitutively under control of a ubiquitin promoter and nopaline synthase terminator. A hygromycin phosphotransferase (hpt) gene is used as a selectable marker, the transgenic line is reported to be strongly resistant to oxyfluorfen, a PPO-inhibiting herbicide
gene LMJF_06_1280, the gene forms a potential transcriptional unit with gene LMJF_06_1270 encoding CPO, coproporphyrinogen III oxidase, EC 1.3.3.3, and with gene LMJF_06_1290 for a cytochrome b5, functional complementation of the Escherichia coli C43(DE3) LG285 DELTAhemG strain, recombinant expression of wild-type and mutant enzymes
gene PPO, structures of the wild-type genomic PPO locus, a T-DNA repair gene-targeting construct, and the targeted PPO locus, overview
-
gene PPO1, recombinant coexpression of GFP-tagged enzyme PPO1 with HA-tagged MORF2 or MORF9 proteins, all under control of the CaMV 35S promoter, in Arabidopsis thaliana, Coexpression of the N-terminal YFP fusion of PPO1 (YFPNPPO1) and the C-terminal YFP fusion of MORF2, MORF9, or MORF8 (i.e. MORF2-YFPC, MORF9-YFPC, orMORF8-YFPC) reconstituting a functional YFP in chloroplasts
gene PPOX, genetic analysis of variegate porphyria, VP, an acute hepatic porphyria, in Italy
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gene PPOX, relative real-time RT-PCR enzyme expression analysis
gene PPX2, recombinant expression in Escherichia coli strain BL21(DE3)-pLysS
in Escherichia coli
in Escherichia coli His-tag inserted for purification
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in Escherichia coli, His-tag inserted for purification
one mutant lacks protoporphyrinogen oxidase activity
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overexpressed in Escherichia coli using the pQE30 expression vector, complementation of Escherichia coli hemG and hemH mutant strains
overexpression in Escherichia coli JM109 cells
overexpression in T2 homozygous transgenic plants under the control of the constitutive maize ubiquitin promoter
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overproduction of mitochondrial isozyme by gene amplification in photomixotrophic cells
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pMx-PPO vector transfected into and maintained in Escherichia coli JM109 cells
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some clones resistant against S-23142-inhibition
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the enzyme gene is fused in its 3'-end to a polynucleotide encoding six histidine residues and expressed in both Escherichia coli BL21(DE3) cells and Bacillus subtilis 168A cells
wild-type and mutant cDNAs cloned into the pEGFP-N1 vector and transiently transfected into HepG2 cells
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