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1.3.7.11: 2,3-bis-O-geranylgeranyl-sn-glycero-phospholipid reductase

This is an abbreviated version!
For detailed information about 2,3-bis-O-geranylgeranyl-sn-glycero-phospholipid reductase, go to the full flat file.

Reaction

a 2,3-bis-(O-phytanyl)-sn-glycero-phospholipid
+ 16 oxidized ferredoxin [iron-sulfur] cluster =
a 2,3-bis-(O-geranylgeranyl)-sn-glycero-phospholipid
 + 16 reduced ferredoxin [iron-sulfur] cluster + 16 H+

Synonyms

AF0464, CrtI homologue, digeranylgeranylglycerophospholipid reductase, EC 1.3.99.34, geranylgeranyl reductase, GGR, MA1484, MA1492, MA_1484, MA_1492, phytoene dehydrogenase family protein, Sa-GGR, SaGGR, Ta0516

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.7 With an iron-sulfur protein as acceptor
                1.3.7.11 2,3-bis-O-geranylgeranyl-sn-glycero-phospholipid reductase

Purification

Purification on EC 1.3.7.11 - 2,3-bis-O-geranylgeranyl-sn-glycero-phospholipid reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strains BLR(DE3) or Rosetta2(DE3) by nickel affinity chromatography, cleavage of the His-tag by TEV protease, followed by another step of nickel affinity chromatography to remove the tag, followed by desalting gel filtration
recombinant N-terminally poly-His-tagged enzyme MA1492 from Escherichia coli strain KRX by cobalt affinity chromatography