1.3.7.4: phytochromobilin:ferredoxin oxidoreductase

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For detailed information about phytochromobilin:ferredoxin oxidoreductase, go to the full flat file.

Reaction

Synonyms

3Z-phytochromobilin:ferredoxin oxidoreductase, At3g09150, AUREA, Csa_1G616870, CsHY2, HT-HY2, HY2, HY2 protein, PFB synthase, phytochromobilin synthase, PphiB synthase, Solyc01 g008930, ZMHy2

ECTree

     1 Oxidoreductases

         1.3 Acting on the CH-CH group of donors

             1.3.7 With an iron-sulfur protein as acceptor

                1.3.7.4 phytochromobilin:ferredoxin oxidoreductase

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4	Activating Compound
17	AA Sequence
3	Application
1	CAS Registry Number
12	Cloned(Commentary)
10	Cofactor
1	Crystallization (Commentary)
1	Expression
13	General Information
3	KM Value [mM]
6	Localization
4	Metals/Ions
4	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
17	Organism
3	Pathway
2	PDB ID
3	pH Optimum
19	Protein Variants
7	Purification (Commentary)
1	Reaction
3	Reaction Type
17	Reference
10	Source Tissue
2	Specific Activity [micromol/min/mg]
16	Substrates and Products (Substrate)
1	Subunits
29	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.3.7.4 - phytochromobilin:ferredoxin oxidoreductase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant wild-type and selenomethionine-labeled phytochromobilin synthase in complex with substrate biliverdin IXalpha, sitting drop vapor diffusion method, mixing of protein in 150 mM KCl, and 20 mM Tris-HCl, pH 7.4, with reservoir solution, at 4°C. Tiny needle-shaped crystals of the BV-PPhiB synthase complex are obtained with a reservoir solution containing 20% w/v PEG 8000, 0.1 M Tris-HCl, pH 8.5, and 0.2 M MgCl2. Isomorphous crystals are also obtained with a reservoir solution containing 30% w/v PEG 4000, 0.1 M Tris-HCl, pH 8.5, and 0.2 M MgCl2, X-ray diffraction structure determination and analysis at 1.95-2.2 A resolution

Solanum lycopersicum

Q588D6

763272