1.3.7.4: phytochromobilin:ferredoxin oxidoreductase

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For detailed information about phytochromobilin:ferredoxin oxidoreductase, go to the full flat file.

Word Map on EC 1.3.7.4

1.3.7.4

litoria

phytochrome

lycoris

calpurnia

alstroemeria

amaryllidaceae

matricaria

tabebuia

phytochrome-deficient

phyllostachys

venerupis

bilins

galanthamine

ethnobotanical

biotechnology

synthesis

Reaction

+ 2 H+

Synonyms

3Z-phytochromobilin:ferredoxin oxidoreductase, At3g09150, AUREA, Csa_1G616870, CsHY2, HT-HY2, HY2, HY2 protein, PFB synthase, phytochromobilin synthase, PphiB synthase, Solyc01 g008930, ZMHy2

ECTree

1 Oxidoreductases

1.3 Acting on the CH-CH group of donors

1.3.7 With an iron-sulfur protein as acceptor

1.3.7.4 phytochromobilin:ferredoxin oxidoreductase

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Results	in table
4	Activating Compound
17	AA Sequence
3	Application
1	CAS Registry Number
12	Cloned(Commentary)
10	Cofactor
1	Crystallization (Commentary)
1	Expression
13	General Information
3	KM Value [mM]
6	Localization
4	Metals/Ions
4	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
17	Organism
3	Pathway
2	PDB ID
3	pH Optimum
19	Protein Variants
7	Purification (Commentary)
1	Reaction
3	Reaction Type
17	Reference
10	Source Tissue
2	Specific Activity [micromol/min/mg]
16	Substrates and Products (Substrate)
1	Subunits
29	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 1.3.7.4 - phytochromobilin:ferredoxin oxidoreductase

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50000fold

90% homogeneity

HPLC Phenomenex Ultracarb reverse phase column chromatography

Arabidopsis thaliana

685125

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion exchange chromatography, and gel filtration. The PPhiB synthase monomer is contained in the major peak of anion-exchange chromatography, and the PPhiB synthase homodimer in the minor peak, for purification, the monomeric major peak fraction is used

recombinant ZmHy2

Superdex 200 gel filtration

Arabidopsis thaliana

687760