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1.3.7.5: phycocyanobilin:ferredoxin oxidoreductase

This is an abbreviated version!
For detailed information about phycocyanobilin:ferredoxin oxidoreductase, go to the full flat file.

Word Map on EC 1.3.7.5

Reaction

(3Z)-phycocyanobilin
+ 4 oxidized ferredoxin =
biliverdin IXalpha
+ 4 reduced ferredoxin

Synonyms

3Z-phycocyanobilin:ferredoxin oxidoreductase, AmPcyAc, AmPcyAp, bilin reductase, FDBR, ferredoxin-dependent biliverdin reductase, ferredoxin:3Z-phycocyanobilin oxidoreductase, HY2 protein, oxidoreductase, ferredoxin:3Z-phycocyanobilin, Pcb:Fd oxidoreductase, PCB:ferredoxin oxidoreductase, PcyA, PCYA1, phycocyanobilin synthase, phycocyanobilin-ferredoxin oxidoreductase, phycocyanobilin:ferredoxin oxidoreductase, SyPcyA

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.7 With an iron-sulfur protein as acceptor
                1.3.7.5 phycocyanobilin:ferredoxin oxidoreductase

Cloned

Cloned on EC 1.3.7.5 - phycocyanobilin:ferredoxin oxidoreductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
Acaryochloris marina strain 11017 encodes two PcyA homologues, AmPcyAc and AmPcyAp. AmPcyAc is encoded on the main chromosome with most photoreceptor genes, whereas AmPcyAp is encoded on a plasmid with PBS-related genes, these PcyA homologues share 56% sequence identity. Cloning of gene pcyA or AM1_5311 and recombinant expression of the His-tagged enzyme in Escherichia coli strain C41, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis
Acaryochloris marina strain 11017 encodes two PcyA homologues, AmPcyAc and AmPcyAp. AmPcyAc is encoded on the main chromosome with most photoreceptor genes, whereas AmPcyAp is encoded on a plasmid with PBS-related genes, these PcyA homologues share 56% sequence identity. Cloning of gene pcyA or AM1_C0185 and recombinant expression of the His-tagged enzyme in Escherichia coli strain C41, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis
cloned into plasmid containing the sequence coding for HO1 protein
-
coexpression in Escherichia coli BL21(DE3) with cpcB(C155I) or pecB(C155I) and with cpeS, ho1 and pcyA, resulting in biosynthesis of the respective beta-subunits singly chromophorylated at cysteine-beta84
-
expression in Arabidopsis thaliana
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expression in Escherichia coli
expression of mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene pcyA, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain C41, coexpression with heme oxygenase 1 (HO1)
gene pycA, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
into vector pET-21a and expressed in Escherichia coli C41(DE3)
PcyA expression in Escherichia coli strain BL21(DE3)
Thermosynechococcus vestitus
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recombinant expression of GST-tagged PcyA in Escherichia coli
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recombinant functional expression of PcyA in Escherichia coli with production of phycocyanobilin, functional co-expression with cyanobacterial heme oxygenase, and the phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview
-
subcloned into pKT210, co-expression with heme oxygenase in Escherichia coli cells
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wild-type and mutants, mutant plasmid constructs expressed in Escherichia coli strain DH5alpha
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