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1.3.8.2: 4,4'-diapophytoene desaturase (4,4'-diapolycopene-forming)

This is an abbreviated version!
For detailed information about 4,4'-diapophytoene desaturase (4,4'-diapolycopene-forming), go to the full flat file.

Word Map on EC 1.3.8.2

Reaction

15-cis-4,4'-diapophytoene
+ 4 FAD =
all-trans-4,4'-diapolycopene
+ 4 FADH2

Synonyms

CrtN, CrtQa, dehydrosqualene desaturase, diapophytoene desaturase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.8 With a flavin as acceptor
                1.3.8.2 4,4'-diapophytoene desaturase (4,4'-diapolycopene-forming)

Cloned

Cloned on EC 1.3.8.2 - 4,4'-diapophytoene desaturase (4,4'-diapolycopene-forming)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpression with dehydroqualene desarurase with dehydrosqualene synthase and induction of endogenous genes crtP and crtQ encoding mixed function oxidase and glycosyltransferase for production of glycosylated C30 carotenoic acid, overview
-
expression in Escherichia coli
gene crtN, functional expression in Bacillus subtilis, coexpression with dehydroqualene synthase and induction of endogenous genes crtP and crtQ
-
recombinant expression of His-tagged enzyme
the carotenoid synthetic genes, crtM and crtN, derived from Staphylococcus aureus, are introduced into Bacillus subtilis, resulting in yellow pigmentation