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1.3.98.1: dihydroorotate dehydrogenase (fumarate)

This is an abbreviated version!
For detailed information about dihydroorotate dehydrogenase (fumarate), go to the full flat file.

Word Map on EC 1.3.98.1

Reaction

(S)-dihydroorotate
+
fumarate
=
Orotate
 +
succinate

Synonyms

(DHO) dehydrogenase, 4,5-L-dihydroorotate:oxygen oxidoreductase, ACT/DHOD, class 1A DHOD, class 1A DHODH, class 1A dihydroorotate dehydrogenase, DHOD, DHOD1, DHOD2, DHOD3, DHODA, DHOdehase, DHODH, DHODH-1A, dihydroorotate dehydrogenase, dihydroorotate dehydrogenase class 1A, Dihydroorotate oxidase, EC 1.3.3.1, LmDHODH, More, oxidase, dihydroorotate, TcDHOD

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.98 With other, known, physiological acceptors
                1.3.98.1 dihydroorotate dehydrogenase (fumarate)

KM Value

KM Value on EC 1.3.98.1 - dihydroorotate dehydrogenase (fumarate)

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00856 - 0.026
(S)-dihydroorotate
0.0131
2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone
at saturating concentration of dihydroorotate
0.115
2,6-dichloroindophenol
-
in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C
0.016 - 0.066
CoQ
0.0192 - 0.071
dihydroorotate
0.001 - 0.344
ferricyanide
0.03 - 0.4135
fumarate
0.0086 - 0.123
L-dihydroorotate
0.001 - 0.5
S-dihydroorotate
additional information
additional information
-
10% v/v dimethyl sulfoxide and 0.5% v/vTriton X-100, which seem to facilitate the substrate binding process with a small decrease in Km, enzyme-substrate complex, pseudo-first-order kinetics, thermodynamics, overview
-