1.3.98.7: [mycofactocin precursor peptide]-tyrosine decarboxylase
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For detailed information about [mycofactocin precursor peptide]-tyrosine decarboxylase, go to the full flat file.
Reaction
Synonyms
mftC, MtfC, mycofactocin maturase, Rv0693
ECTree
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General Information
General Information on EC 1.3.98.7 - [mycofactocin precursor peptide]-tyrosine decarboxylase
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physiological function
a MftC deletion mutant is unable to grow in minimal medium containing cholesterol (0.01% [wt/vol]) as the sole carbon source with hot ethanol (1% [vol/vol]) as the solvent (cholesterol and EtOH [cholesterol:EtOH]). There is no significant growth retardation in cholesterol:DMSO. Supplementation of ethanol has no effect on mutant strain growth in propionate (0.5% [wt/vol]) or glycerol (0.2% [vol/vol]) but severely delays mutant strain growth in acetate (0.5% [wt/vol]) in comparison with the growth of the wild-type strain. MftC deletion results in multifold increases in expression of the DosR regulon genes in ethanol-treated strains
physiological function
MtfC is a radical SAM enzyme and oxidatively decarboxylates the C-terminus of the MftA peptide in the presence of the accessory protein MftB. MftC abstracts a hydrogen atom from the beta-carbon of the C-terminal Tyr residue. The resulting radical species is stabilized by the adjacent phenol ring. Decarboxylation occurs either via transfer of the unpaired spin from the radical intermediate to a [4Fe-4S] cluster concomitant with decarboxylation to form the final product. Alternatively, the Calpha-COOH bond can be homolytically cleaved resulting in the formation of a COOH radical species that can either be quenched to formate or CO2
physiological function
radical SAM protein Rv0693 is involved in biosynthesis of cofactor mycofactocin and targets a small peptide for modification
physiological function
-
a MftC deletion mutant is unable to grow in minimal medium containing cholesterol (0.01% [wt/vol]) as the sole carbon source with hot ethanol (1% [vol/vol]) as the solvent (cholesterol and EtOH [cholesterol:EtOH]). There is no significant growth retardation in cholesterol:DMSO. Supplementation of ethanol has no effect on mutant strain growth in propionate (0.5% [wt/vol]) or glycerol (0.2% [vol/vol]) but severely delays mutant strain growth in acetate (0.5% [wt/vol]) in comparison with the growth of the wild-type strain. MftC deletion results in multifold increases in expression of the DosR regulon genes in ethanol-treated strains
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physiological function
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MtfC is a radical SAM enzyme and oxidatively decarboxylates the C-terminus of the MftA peptide in the presence of the accessory protein MftB. MftC abstracts a hydrogen atom from the beta-carbon of the C-terminal Tyr residue. The resulting radical species is stabilized by the adjacent phenol ring. Decarboxylation occurs either via transfer of the unpaired spin from the radical intermediate to a [4Fe-4S] cluster concomitant with decarboxylation to form the final product. Alternatively, the Calpha-COOH bond can be homolytically cleaved resulting in the formation of a COOH radical species that can either be quenched to formate or CO2
-
physiological function
-
radical SAM protein Rv0693 is involved in biosynthesis of cofactor mycofactocin and targets a small peptide for modification
-
physiological function
-
radical SAM protein Rv0693 is involved in biosynthesis of cofactor mycofactocin and targets a small peptide for modification
-