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1.3.99.36: cypemycin cysteine dehydrogenase (decarboxylating)

This is an abbreviated version!
For detailed information about cypemycin cysteine dehydrogenase (decarboxylating), go to the full flat file.

Reaction

cypemycin(1-18)-L-Cys-L-Leu-L-Val-L-Cys
+
acceptor
=
C3.19,S21-cyclocypemycin(1-18)-L-Ala-L-Leu-N-thioethenyl-L-valinamide
 +
CO2
 +
H2S
 +
reduced acceptor

Synonyms

CYPD, cypemycin decarboxylase, cypemycin dehydratase.

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.99 With unknown physiological acceptors
                1.3.99.36 cypemycin cysteine dehydrogenase (decarboxylating)

Purification

Purification on EC 1.3.99.36 - cypemycin cysteine dehydrogenase (decarboxylating)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration
-
recombinant SeMet-substituted CypD from Escherichia coli strain BL21(DE3)
-