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1.3.99.4: 3-oxosteroid 1-dehydrogenase

This is an abbreviated version!
For detailed information about 3-oxosteroid 1-dehydrogenase, go to the full flat file.

Word Map on EC 1.3.99.4

Reaction

a 3-oxosteroid
+
acceptor
=
a 3-oxo-DELTA1-steroid
+
reduced acceptor

Synonyms

1-ene-dehydrogenase, 3-keto-5alpha-steroid DELTA(1)-dehydrogenase, 3-ketosteroid 1(2)-dehydrogenase, 3-ketosteroid delta1-dehydrogenase, 3-ketosteroid-1-en-dehydrogenase, 3-ketosteroid-D1-dehydrogenase, 3-ketosteroid-DELTA1-dehydrogenase, 3-ketosteroid-DELTA1-dehydrogenases, 3-oxosteroid D1-dehydrogenase, 3-oxosteroid Delta1-dehydrogenase, 3-oxosteroid:(2,6-dichlorphenolindophenol) DELTA1-oxidoreductase, 3-oxosteroid:(2,6-dichlorphenolindophenol)D1-oxidoreductase, 4-en-3-oxosteroid:(acceptor)-1-en-oxido-reductase, 4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase, 4-ene-3-oxosteroid:(acceptor)l-ene-oxidoreductase, AcmB, AcmB2, cholest-4-en-3-one DELTA1-dehydrogenase, D1-dehydrogenase, D1-steroid reductase, dehydrogenase, 3-oxo steroid Delta 1-, Delta1-dehydrogenase, DELTA1-KstD, DELTA1-KSTD1, DELTA1-KstDA, DELTA1-KstDR, DELTA1-steroid reductase, ketosteroid DELTA1-dehydrogenase, KsdD, KsdD1, KsdD2, KsdD3, KsdD5, KsdDM, KSDH, KstD, KSTD1, KSTD2, KSTD3, KSTD3 orthologue, KstDF, MnKstD1, MnKstD2, MsKstD1, MsKstD2, MsKstD3, MsKstD4, ReKstD1, Rv3537, steroid-1-dehydrogenase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.99 With unknown physiological acceptors
                1.3.99.4 3-oxosteroid 1-dehydrogenase

Crystallization

Crystallization on EC 1.3.99.4 - 3-oxosteroid 1-dehydrogenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with the reaction product 1,4-androstadiene-3,17-dione, to 2.3 A resolution. Key active site residues are Tyr119, Tyr318, Tyr487, and Gly491
sitting drop vapour diffusion method, mixing of 15 mg/ml protein in 25 mM bicine, pH 9.0, 100 mM NaCl, 10% v/v glycerol with 2% v/v PEG 400, 0.1 M, HEPES, pH 7.5, 2.0 M ammonium sulfate, 20°C, 5-7 days, method optimization, X-ray diffraction structure determination and analysis at 2.0 A resolution, multiwavelength anomalous dispersion analysis