1.4.1.13: glutamate synthase (NADPH)

This is an abbreviated version!
For detailed information about glutamate synthase (NADPH), go to the full flat file.

Word Map on EC 1.4.1.13

Reaction

2 L-glutamate +

NADP+
=
L-glutamine
+
2-oxoglutarate
+
NADPH
+
H+

Synonyms

EC 2.6.1.53, EhNO1, EhNO2, gltA, gltB, GltB1, GltB2, gltD, GltS, glutamate synthetase (NADP), glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP), glutamine-ketoglutaric aminotransferase, GOGAT, L-glutamate synthase, L-glutamate synthetase, L-glutamine:2-oxoglutarate aminotransferase, NADPH oxidizing, NADPH-dependent glutamate synthase, NADPH-GltS, NADPH-glutamate synthase, NADPH-GOGAT, NADPH-linked glutamate synthase, pGLTY1, pGLTY2, pGLTZ, PH0876, PH1873, synthase, glutamate (reduced nicotinamide adenine dinucleotide phosphate)

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.13 glutamate synthase (NADPH)

General Stability

General Stability on EC 1.4.1.13 - glutamate synthase (NADPH)

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2 mM alpha-ketoglutarate, 10 mM beta-mercaptoethanol and 10% glycerol when present together markedly enhance stability
-
2-mercaptoethanol, stabilizes
alpha-ketoglutarate stabilizes
alpha-ketoglutarate, L-glutamine, L-glutamate and NADP when present together partial protect against enzyme inactivation
-
DTT, 1 mM, stabilizes at 4C
-
EDTA, stabilizes
glutamine stabilizes
incubation with L-glutamine or L-glutamate results in marked time- and temperature-dependent loss in activity
-
NADP causes relatively small loss in activity compared with control values
-
unprotected enzyme is unstable, losing 65% of its activity in 24 hours at 4C
-