1.4.1.13: glutamate synthase (NADPH)

This is an abbreviated version!
For detailed information about glutamate synthase (NADPH), go to the full flat file.

Word Map on EC 1.4.1.13

Reaction

2 L-glutamate +

NADP+
=
L-glutamine
+
2-oxoglutarate
+
NADPH
+
H+

Synonyms

EC 2.6.1.53, EhNO1, EhNO2, gltA, gltB, GltB1, GltB2, gltD, GltS, glutamate synthetase (NADP), glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP), glutamine-ketoglutaric aminotransferase, GOGAT, L-glutamate synthase, L-glutamate synthetase, L-glutamine:2-oxoglutarate aminotransferase, NADPH oxidizing, NADPH-dependent glutamate synthase, NADPH-GltS, NADPH-glutamate synthase, NADPH-GOGAT, NADPH-linked glutamate synthase, pGLTY1, pGLTY2, pGLTZ, PH0876, PH1873, synthase, glutamate (reduced nicotinamide adenine dinucleotide phosphate)

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.13 glutamate synthase (NADPH)

Reference

Reference on EC 1.4.1.13 - glutamate synthase (NADPH)

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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Miller, R.E.; Stadtman, E.R.
Glutamate synthase from Escherichia coli. An iron-sulfide flavoprotein
J. Biol. Chem.
247
7407-7419
1972
Escherichia coli
Manually annotated by BRENDA team
Tempest, D.W.; Meers, J.L.
Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route
Biochem. J.
117
405-407
1970
Klebsiella aerogenes
Manually annotated by BRENDA team
Mei, B.; Jiao, R.
Purification and properties of glutamate synthase from Nocardia mediterranei
J. Bacteriol.
170
1940-1944
1988
Amycolatopsis mediterranei
Manually annotated by BRENDA team
Schmidt, C.N.G.; Jervis, L.
Affinity purification of glutamate synthase from Escherichia coli
Anal. Biochem.
104
127-129
1980
Escherichia coli
Manually annotated by BRENDA team
Rendina, A.R.; Orme-Johnson, W.H.
Glutamate synthase: on the kinetic mechanism of the enzyme from Escherichia coli W
Biochemistry
17
5388-5393
1978
Escherichia coli
Manually annotated by BRENDA team
Janssen, D.B.; op den Camp, H.J.M.; Leenen, P.J.M.; van der Drift, C.
The enzymes of the ammonia assimilation in Pseudomonas aeruginosa
Arch. Microbiol.
124
197-203
1980
Pseudomonas aeruginosa
Manually annotated by BRENDA team
Vanoni, M.A.; Edmondson, D.E.; Zanetti, G.; Curti, B.
Characterization of the flavins and the iron-sulfur centers of glutamate synthase from Azospirillum brasilense by absorption, circular dichroism, and electron paramagnetic resonance spectroscopies
Biochemistry
31
4613-4623
1992
Azospirillum brasilense
Manually annotated by BRENDA team
Vanoni, M.A.; Edmondson, D.E.; Rescigno, M.; Zanetti, G.; Curti, B.
Mechanistic studies on Azospirillum brasilense glutamate synthase
Biochemistry
30
11478-11484
1991
Azospirillum brasilense
Manually annotated by BRENDA team
Ratti, S.; Curti, B.; Zanetti, G.; Galli, E.
Purification and characterization of glutamate synthase from Azospirillum brasilense
J. Bacteriol.
163
724-729
1985
Azospirillum brasilense
Manually annotated by BRENDA team
Sung, H.C.; Tachiki, T.; Kumagai, H.; Tochikura, T.
Properties of glutamate synthase from Brevibachterium flavum
J. Ferment. Technol.
62
569-575
1984
Brevibacterium flavum
-
Manually annotated by BRENDA team
Tachiki, T.; Sung, H.C.; Wakisaka, S.; Tochikura, T.
Purification and some properties of glutamate synthase from Gluconobacter subodydans on glutamate as a nitrogen source
J. Ferment. Technol.
61
179-184
1983
Gluconobacter oxydans
-
Manually annotated by BRENDA team
Schreier, H.J.; Bernlohr, R.W.
Purification and properties of glutamate synthase from Bacillus licheniformis
J. Bacteriol.
160
591-599
1984
Bacillus licheniformis
Manually annotated by BRENDA team
Suzuki, A.; Jacquot, J.P.; Gadal, P.
Glutamate synthase in rice roots. Studies on the electron donor specificity
Phytochemistry
22
1543-1546
1983
Oryza sativa
-
Manually annotated by BRENDA team
Schmidt, C.N.G.; Jervis, L.
Partial purification and characterization of glutamate synthase from a thermophilic bacillus
J. Gen. Microbiol.
128
1713-1718
1982
Geobacillus stearothermophilus
-
Manually annotated by BRENDA team
Yelton, M.M.; Yoch, D.C.
Nitrogen metabolism in Rhodospirillum rubrum: Characterization of glutamate synthase
J. Gen. Microbiol.
123
335-342
1981
Rhodospirillum rubrum
-
Manually annotated by BRENDA team
Nisbet, B.A.; Slaughter, J.C.
Glutamate dehydrogenase and glutamate synthase from the yeast Kluyveromyces fragilis: Variability in occurrence and properties
FEMS Microbiol. Lett.
7
319-321
1980
Kluyveromyces marxianus
-
Manually annotated by BRENDA team
Hemmilä, I.A.; Mäntsälä, P.I.
Purification and properties of glutamate synthase and glutamate dehydrogenase from Bacillus megaterium
Biochem. J.
173
45-52
1978
Bacillus megaterium
Manually annotated by BRENDA team
Adachi, K.; Suzuki, I.
Purification and properties of glutamate synthase from Thiobacillus thioparus
J. Bacteriol.
129
1173-1182
1977
Thiobacillus thioparus
Manually annotated by BRENDA team
Mäntsälä, P.; Zalkin, H.
Properties of apoglutamate synthase and comparison with glutamate dehydrogenase
J. Biol. Chem.
251
3300-3305
1976
Escherichia coli
Manually annotated by BRENDA team
Mäntsälä, P.; Zalkin, H.
Glutamate synthase. Properties of the glutamine-dependent activity
J. Biol. Chem.
251
3294-3299
1976
Escherichia coli
Manually annotated by BRENDA team
Hua, S.S.T.; Lichens, G.M.; Guirao, A.; Tsai, V.Y.
Biochemical properties of glutamate synthase of salt-tolerant Bradyrhizobium sp. Strain WR1001
FEMS Microbiol. Lett.
37
209-213
1986
Bradyrhizobium sp.
-
Manually annotated by BRENDA team
Matsuoka, K.; Kimura, K.
Glutamate synthase from Bacillus subtilis PCI 219
J. Biochem.
99
1087-1100
1986
Bacillus subtilis
Manually annotated by BRENDA team
Meister, A.
Glutamate synthase from Escherichia coli, Klebsiella aerogenes, and Saccharomyces cerevisiae
Methods Enzymol.
113
327-337
1985
Escherichia coli, Klebsiella aerogenes
Manually annotated by BRENDA team
Rachim, M.A.; Nicholas, D.J.D.
Glutamine synthetase and glutamate synthase from Sclerotinia Schlerotiorum
Phytochemistry
24
2541-2548
1985
Sclerotinia sclerotiorum
-
Manually annotated by BRENDA team
Carlberg, I.; Norlund, S.
Purification and partial characterization of glutamate synthase from Rhodospirillum rubrum grown under nitrogen-fixing conditions
Biochem. J.
279
151-154
1991
Rhodospirillum rubrum
Manually annotated by BRENDA team
Vanoni, M.A.; Verzotti, E.; Zanetti, G.; Curti, B.
Properties of the recombinant beta subunit of glutamate synthase
Eur. J. Biochem.
236
937-946
1996
Azospirillum brasilense
Manually annotated by BRENDA team
Jongsareejit, B.; Rahman, R.N.Z.A.; Fujiwara, S.; Imanaka, T.
Gene cloning, sequencing and enzymic properties of glutamate synthase from the hyperthermophilic archaeon Pyrococcus sp. KOD1
Mol. Gen. Genet.
254
635-642
1997
Pyrococcus sp.
Manually annotated by BRENDA team
Vanoni, M.A.; Fischer, F.; Ravasio, S.; Verzotti, E.; Edmondson, D.E.; Hagen, W.R.; Zanetti, G.; Curti, B.
The recombinant alpha subunit of glutamate synthase: spectroscopic and catalytic properties
Biochemistry
37
1828-1838
1998
Azospirillum brasilense
Manually annotated by BRENDA team
Morandi, P.; Valzasina, B.; Colombo, C.; Curti, B.; Vanoni, M.A.
Glutamate Synthase: Identification of the NADPH-Binding Site by Site-Directed Mutagenesis
Biochemistry
39
727-735
2000
Azospirillum brasilense
Manually annotated by BRENDA team
Stabile, H.; Curti, B.; Vanoni, M.A.
Functional properties of recombinant Azospirillum brasilense glutamate synthase, a complex iron-sulfur flavoprotein
Eur. J. Biochem.
267
2720-2730
2000
Azospirillum brasilense
Manually annotated by BRENDA team
Ravasio, S.; Curti, B.; Vanoni, M.A.
Determination of the Midpoint Potential of the FAD and FMN Flavin Cofactors and of the 3Fe-4S Cluster of Glutamate Synthase
Biochemistry
40
5533-5541
2001
Azospirillum brasilense
Manually annotated by BRENDA team
Ravasio, S.; Dossena, L.; Martin-Figueroa, E.; Florencio, F.J.; Mattevi, A.; Morandi, P.; Curti, B.; Vanoni, M.A.
Properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803. Comparison with the Azospirillum brasilense NADPH-dependent enzyme and its isolated alpha subunit
Biochemistry
41
8120-8133
2002
Azospirillum brasilense
Manually annotated by BRENDA team
Agnelli, P.; Dossena, L.; Colombi, P.; Mulazzi, S.; Morandi, P.; Tedeschi, G.; Negri, A.; Curti, B.; Vanoni, M.A.
The unexpected structural role of glutamate synthase [4Fe-4S](+1,+2) clusters as demonstrated by site-directed mutagenesis of conserved C residues at the N-terminus of the enzyme beta subunit
Arch. Biochem. Biophys.
436
355-366
2005
Azospirillum brasilense
Manually annotated by BRENDA team
Stutz, H.E.; Reid, S.J.
GltX from Clostridium saccharobutylicum NCP262: glutamate synthase or oxidoreductase?
Biochim. Biophys. Acta
1676
71-82
2004
Clostridium saccharobutylicum (Q841S7), Clostridium saccharobutylicum NCP262 (Q841S7)
Manually annotated by BRENDA team
Petoukhov, M.V.; Svergun, D.I.; Konarev, P.V.; Ravasio, S.; van den Heuvel, R.H.; Curti, B.; Vanoni, M.A.
Quaternary structure of Azospirillum brasilense NADPH-dependent glutamate synthase in solution as revealed by synchrotron radiation x-ray scattering
J. Biol. Chem.
278
29933-29939
2003
Azospirillum brasilense, Azospirillum brasilense (Q05755)
Manually annotated by BRENDA team
Coiro, V.M.; Di Nola, A.; Vanoni, M.A.; Aschi, M.; Coda, A.; Curti, B.; Roccatano, D.
Molecular dynamics simulation of the interaction between the complex iron-sulfur flavoprotein glutamate synthase and its substrates
Protein Sci.
13
2979-2991
2004
Azospirillum brasilense
Manually annotated by BRENDA team
Saum, S.H.; Sydow, J.F.; Palm, P.; Pfeiffer, F.; Oesterhelt, D.; Mueller, V.
Biochemical and molecular characterization of the biosynthesis of glutamine and glutamate, two major compatible solutes in the moderately halophilic bacterium Halobacillus halophilus
J. Bacteriol.
188
6808-6815
2006
Halobacillus halophilus, Halobacillus halophilus (Q0E5H3), Halobacillus halophilus (Q0E5H4), Halobacillus halophilus (Q0E5H5)
Manually annotated by BRENDA team
Chung, T.W.; Lee, D.I.; Kim, D.S.; Jin, U.H.; Park, C.; Kim, J.G.; Kim, M.G.; Ha, S.D.; Kim, K.S.; Lee, K.H.; Kim, K.Y.; Chung, D.H.; Kim, C.H.
Molecular cloning and characterization of a large subunit of Salmonella typhimurium glutamate synthase (GOGAT) gene in Escherichia coli
J. Microbiol.
44
301-310
2006
Salmonella enterica subsp. enterica serovar Typhimurium (Q9L694), Salmonella enterica subsp. enterica serovar Typhimurium
Manually annotated by BRENDA team
Suzuki, A.; Knaff, D.B.
Glutamate synthase: structural, mechanistic and regulatory properties, and role in the amino acid metabolism
Photosyn. Res.
83
191-217
2005
Acidithiobacillus ferrooxidans (Q56266), Acidithiobacillus ferrooxidans (Q56267), Archaeoglobus fulgidus (O29309), Azospirillum brasilense (Q05755), Azospirillum brasilense (Q05756), Azospirillum brasilense (Q59084), Bacillus megaterium, Bacillus subtilis (P39812), Escherichia coli (P09831), Escherichia coli (P09832), Klebsiella aerogenes, Methanocaldococcus jannaschii (Q58746), Methanococcus thermoautotrophicum (O26308), no activity in Arabidopsis thaliana, Pseudomonas aeruginosa (P95456), Pseudomonas aeruginosa (P95457), Pyrococcus sp., Pyrococcus sp. KOD1, Rhizobium etli (Q9ZFB8), Rhizobium etli (Q9ZFB9), Rhodospirillum rubrum, Salmonella enterica subsp. enterica serovar Typhimurium (Q8ZLR3), Salmonella enterica subsp. enterica serovar Typhimurium (Q8ZLR4)
Manually annotated by BRENDA team
Vanoni, M.A.; Dossena, L.; van den Heuvel, R.H.; Curti, B.
Structure-function studies on the complex iron-sulfur flavoprotein glutamate synthase: the key enzyme of ammonia assimilation
Photosyn. Res.
83
219-238
2005
Azospirillum brasilense
Manually annotated by BRENDA team
Hemmilä, I.A.; Mäntsälä, P.I.
Inactivation of glutamate dehydrogenase and glutamate synthase from Bacillus megaterium by phenylglyoxal, butane-2,3-dione and pyridoxal 5'-phosphate
Biochem. J.
173
53-58
1978
Bacillus megaterium
Manually annotated by BRENDA team
Cottevieille, M.; Larquet, E.; Jonic, S.; Petoukhov, M.V.; Caprini, G.; Paravisi, S.; Svergun, D.I.; Vanoni, M.A.; Boisset, N.
The subnanometer resolution structure of the glutamate synthase 1.2-MDa hexamer by cryoelectron microscopy and its oligomerization behavior in solution: functional implications
J. Biol. Chem.
283
8237-8249
2008
Azospirillum brasilense (Q05756)
Manually annotated by BRENDA team
Jeelani, G.; Husain, A.; Sato, D.; Ali, V.; Suematsu, M.; Soga, T.; Nozaki, T.
Two atypical L-cysteine-regulated NADPH-dependent oxidoreductases involved in redox maintenance, L-cystine and iron reduction, and metronidazole activation in the enteric protozoan Entamoeba histolytica
J. Biol. Chem.
285
26889-26899
2010
Entamoeba histolytica (C4LUA1), Entamoeba histolytica (C8KIP3), Entamoeba histolytica, Entamoeba histolytica HM1:IMSS cl 6 (C4LUA1), Entamoeba histolytica HM1:IMSS cl 6 (C8KIP3)
Manually annotated by BRENDA team
Dincturk, H.B.; Cunin, R.; Akce, H.
Expression and functional analysis of glutamate synthase small subunit-like proteins from archaeon Pyrococcus horikoshii
Microbiol. Res.
166
294-303
2011
Pyrococcus horikoshii (A0A171), Pyrococcus horikoshii (B3FYT6), Pyrococcus horikoshii
Manually annotated by BRENDA team
Dincturk, H.; Cunin, R.; Akce, H.
Expression and functional analysis of glutamate synthase small subunit-like proteins from archaeon Pyrococcus horikoshii
Microbiol. Res.
166
294-303
2011
Pyrococcus horikoshii (O58606), Pyrococcus horikoshii, Pyrococcus horikoshii OT-3 (O58606), Pyrococcus horikoshii OT3 (O59547)
Manually annotated by BRENDA team