1.4.1.13: glutamate synthase (NADPH)

This is an abbreviated version!
For detailed information about glutamate synthase (NADPH), go to the full flat file.

Word Map on EC 1.4.1.13

Reaction

2 L-glutamate +

NADP+
=
L-glutamine
+
2-oxoglutarate
+
NADPH
+
H+

Synonyms

EC 2.6.1.53, EhNO1, EhNO2, gltA, gltB, GltB1, GltB2, gltD, GltS, glutamate synthetase (NADP), glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP), glutamine-ketoglutaric aminotransferase, GOGAT, L-glutamate synthase, L-glutamate synthetase, L-glutamine:2-oxoglutarate aminotransferase, NADPH oxidizing, NADPH-dependent glutamate synthase, NADPH-GltS, NADPH-glutamate synthase, NADPH-GOGAT, NADPH-linked glutamate synthase, pGLTY1, pGLTY2, pGLTZ, PH0876, PH1873, synthase, glutamate (reduced nicotinamide adenine dinucleotide phosphate)

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.13 glutamate synthase (NADPH)

Temperature Stability

Temperature Stability on EC 1.4.1.13 - glutamate synthase (NADPH)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-20
-
complete loss of activity after 10 days
-10
-
stable for at least 6 weeks
0
-
stable for 6-8 h in crude extract
4
-
unprotected enzyme loses 90% of activity in 48 hours, protected enzyme, addition of 5 mM EDTA and 5 mM 2-mercaptoethanol or 1 mM DTT retains 80 to 90% of the activity after 48 hours
40
-
10 min, 80% loss of activity, enzyme in crude extract
55
-
10 min, in 0.1 M potassium phosphate buffer, pH 6.8, 70-80% loss of activity
70 - 100
purified enzyme, 10 min, stable up to 90C; purified enzyme, 10 min, stable up to 90C