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1.4.1.18: lysine 6-dehydrogenase

This is an abbreviated version!
For detailed information about lysine 6-dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.18

Reaction

L-lysine
+
NAD+
+
H2O
=
(S)-2-amino-6-oxohexanoate
+
NADH
+
H+
+
NH3

Synonyms

L-lysine 6-dehydrogenase, L-lysine 6-dehydrogenase (deaminating), L-lysine epsilon-dehydrogenase, Lys 6-DH, LysDH, lysine 6-dehydrogenase

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.18 lysine 6-dehydrogenase

Engineering

Engineering on EC 1.4.1.18 - lysine 6-dehydrogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F173A
mutation creates an additional large hydrophobic binding pocket in the active site, which favours the binding of aromatic substrates
F173G
very low level of activity towards the tested substrates
R242M
residue R242 appears to be involved in the binding of the alpha-carboxylic group of L-lysine and is located at the entrance of the active site
R242M/F173A
mutant converts substrates 1-phenylethan-1-one (6%) and 1-phenylpropan-1-one (19%, ee > 99% R)
R242M/F173V
mutant converts substrates 1-phenylethan-1-one, 1-phenylpropan-1-one 1-phenylbutan-1-one and 2,3-dihydro-4H-1-benzopyran-4-one (38%, 41%, 8%, 15% conversions, ee > 99% R)
V130A/F173A
mutant exhibits a significant level of activity towards 1-phenylethan-1-one (74% conversion) and 1-phenylpropan-1-one (65% conversion), and all amine products are obtained in enantiomerically pure form (ee > 99% R)
V130G/F173A
mutant is stereospecific (ee > 99% R) for the amination of 1-phenylpropan-1-one (78% conversion) and other ketones
V172A/F173A
dramatic reduction of catalytic activity
V172A/F173G
very low level of activity towards the tested substrates
V172G/F173A
dramatic reduction of catalytic activity
V172G/F173G
very low level of activity towards the tested substrates
E168A
site-directed mutagenesis
E168Q
site-directed mutagenesis
R226K
site-directed mutagenesis
Y156F
site-directed mutagenesis
E168A
-
site-directed mutagenesis
-
E168Q
-
site-directed mutagenesis
-
R226K
-
site-directed mutagenesis
-
Y156F
-
site-directed mutagenesis
-