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1.4.1.18: lysine 6-dehydrogenase

This is an abbreviated version!
For detailed information about lysine 6-dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.18

Reaction

L-lysine
+
NAD+
+
H2O
=
(S)-2-amino-6-oxohexanoate
+
NADH
+
H+
+
NH3

Synonyms

L-lysine 6-dehydrogenase, L-lysine 6-dehydrogenase (deaminating), L-lysine epsilon-dehydrogenase, Lys 6-DH, LysDH, lysine 6-dehydrogenase

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.18 lysine 6-dehydrogenase

Purification

Purification on EC 1.4.1.18 - lysine 6-dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme is purified approximately 100fold to homogeneity with a 15% yield
native LysDH, SuperQ-Toyopearl, Red-Sepharose, Gigapite, Cellulofine, recombinant LysDH, Red-Sepharose
refolded recombinant His-tagged wild-type and mutant LysDHs from Escherichia coli strain Rosettagami (DE3) by gel filtration and nickel affinity chromatography