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2.8
2-oxo-4-methyl-thiobutanoate
-
wild-type enzyme
13 - 50
2-oxo-4-methylpentanoate
2.1 - 87
2-oxo-4-methylthiobutanoate
9.1 - 56
2-Oxoisohexanoate
0.25 - 110
3-acetylpyridine-NAD+
50
3-pyridinealdehyde-NAD+
-
-
0.24 - 1.3
4-hydroxyphenylpyruvate
20
4-methyl-2-oxopentanoate
-
25°C, pH 10.4
1.4
4-phenyl-2-butanone
mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C
0.45
4-trifluoromethyl-phenylpyruvate
-
mutant enzyme N145A, at 25°C inTris buffer 50mM pH 8.5, with NADH (0.1 mM), KCl (100 mM) and NH4Cl (400 mM)
11
alpha-keto-gamma-methiobutyrate
-
25°C, pH 10.4
13
alpha-Ketocaproate
-
25°C, pH 10.4
52
alpha-ketovalerate
-
25°C, pH 10.4
13
DL-alpha-keto-beta-methyl-n-valerate
-
25°C, pH 10.4
2 - 298
DL-propargylglycine
0.14
L-isoleucine
-
25°C, pH 10.4
0.55
L-leucine
-
25°C, pH 10.4
2.7
L-methionine
-
25°C, pH 10.4
0.018 - 9.6
L-phenylalanine
2
L-valine
-
25°C, pH 10.4
0.22
norleucine
-
wild-type enzyme
170
oxidized beta-nicotinamide guanine dinucleotide
-
-
40
oxidized beta-nicotinamide hypoxanthine dinucleotide
-
-
0.19
p-hydroxy-phenylpyruvate
-
25°C, pH 10.4
2.4
p-hydroxyphenylpyruvate
-
-
4
phenylacetone
mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C
0.052 - 23
phenylpyruvate
0.18
thionicotinamide-NAD+
-
-
13
2-oxo-4-methylpentanoate
-
wild-type enzyme
50
2-oxo-4-methylpentanoate
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
2.1
2-oxo-4-methylthiobutanoate
-
-
87
2-oxo-4-methylthiobutanoate
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
2.44
2-Oxohexanoate
-
-
2.5
2-Oxohexanoate
-
wild-type enzyme
47
2-Oxohexanoate
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
9.1
2-Oxoisohexanoate
-
wild-type enzyme
56
2-Oxoisohexanoate
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
35
2-oxopentanoate
-
wild-type enzyme
107
2-oxopentanoate
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
0.25
3-acetylpyridine-NAD+
-
-
110
3-acetylpyridine-NAD+
-
-
0.24
4-hydroxyphenylpyruvate
-
-
0.34
4-hydroxyphenylpyruvate
-
-
1.3
4-hydroxyphenylpyruvate
-
-
2 - 3
DL-propargylglycine
-
mutations G124A/E313G
28
DL-propargylglycine
-
mutation E313G
28
DL-propargylglycine
-
mutation G124A
32
DL-propargylglycine
-
mutations K224Q/L283F/E313G
32
DL-propargylglycine
-
mutations Q18H/I336F
34
DL-propargylglycine
-
mutations K224Q/L283F
39
DL-propargylglycine
-
mutations Q18H/I336F/E313G
42
DL-propargylglycine
-
mutations F110Y/G124C/G293A
44
DL-propargylglycine
-
mutations V33A/A206D/L283F
48
DL-propargylglycine
-
mutations G124A/E313N
58
DL-propargylglycine
-
mutations D209G/Q18H/I336F
84
DL-propargylglycine
-
mutations V135I/I308N/Q363H
134
DL-propargylglycine
-
wild type enzyme of Bacillus sphaericus
298
DL-propargylglycine
-
mutation D209G
0.27
L-ethionine
-
wild-type enzyme
4
L-ethionine
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
0.09
L-Ile
-
wild-type enzyme
10
L-Ile
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
0.06
L-Leu
-
chimeric enzyme
0.09
L-Leu
-
wild-type enzyme
4.7
L-Leu
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
0.34
L-Met
-
wild-type enzyme
7.5
L-Met
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
0.79
L-norleucine
-
25°C, pH 10.4
2.1
L-norleucine
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
0.53
L-norvaline
-
wild-type enzyme
3.3
L-norvaline
-
25°C, pH 10.4
10
L-norvaline
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
0.09
L-Phe
-
mutant enzyme K81A
0.1
L-Phe
-
wild-type enzyme
0.12
L-Phe
-
chimeric enzyme
0.164
L-Phe
-
25°C, pH 10.4, dextran-modified enzyme
0.186
L-Phe
-
PheDH after glycosidation
0.2
L-Phe
-
in 100 mM glycine-KCl-KOH buffer (pH 10.4)
0.21
L-Phe
-
native enzyme, at 25°C, in 100 mM glycine/KCl/KOH buffer pH 10.4
0.215
L-Phe
-
25°C, pH 10.4, native enzyme
0.215
L-Phe
-
unmodified PheDH
20
L-Phe
-
mutant enzyme K69A/K81A
23
L-Phe
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
24
L-Phe
-
immobilized enzyme, at 25°C, in 100 mM glycine/KCl/KOH buffer pH 10.4
140
L-Phe
-
mutant enzyme K69A
0.018
L-phenylalanine
-
mutations Q18H/I336F/E313G
0.02
L-phenylalanine
-
mutations K224Q/L283F/E313G
0.035
L-phenylalanine
-
mutations V135I/I308N/Q363H
0.038
L-phenylalanine
-
pH 10.4, 30°C, immobilized enzyme
0.04
L-phenylalanine
-
mutations K224Q/L283F
0.04
L-phenylalanine
mutant V144L, pH 10.4, 25°C
0.052
L-phenylalanine
-
pH 10.4, 30°C, native enzyme
0.06
L-phenylalanine
-
mutations V33A/A206D/L283F
0.06
L-phenylalanine
mutant V144D, pH 10.4, 25°C
0.063
L-phenylalanine
-
pH 10.4, 25°C, immobilized enzyme
0.07
L-phenylalanine
-
mutations D209G/Q18H/I336F
0.07
L-phenylalanine
-
mutations Q18H/I336F
0.076
L-phenylalanine
-
pH 10.4, 30°C, immobilized enzyme
0.08
L-phenylalanine
wild-type, pH 10.4, 25°C
0.087
L-phenylalanine
-
-
0.118
L-phenylalanine
-
pH 10.4, 25°C, native enzyme
0.12
L-phenylalanine
-
mutation E313G
0.167
L-phenylalanine
-
pH 10.4, 30°C, native enzyme
0.19
L-phenylalanine
mutant V144N, pH 10.4, 25°C
0.67
L-phenylalanine
-
mutation D209G
1.1
L-phenylalanine
-
25°C, pH 10.4
1.4
L-phenylalanine
-
wild type enzyme of Bacillus sphaericus
1.51
L-phenylalanine
-
mutations F110Y/G124C/G293A
4.5
L-phenylalanine
-
mutations G124A/E313N
7.9
L-phenylalanine
-
mutation G124A
9.6
L-phenylalanine
-
mutations G124A/E313G
1.1
L-Tyr
-
-
0.48
L-tyrosine
-
-
1.3
L-tyrosine
-
25°C, pH 10.4
4.7
L-tyrosine
mutant V144N, pH 10.4, 25°C
8.6
L-tyrosine
wild-type, pH 10.4, 25°C
11.9
L-tyrosine
mutant V144L, pH 10.4, 25°C
27
L-tyrosine
mutant V144D, pH 10.4, 25°C
0.057
NAD+
-
mutant enzyme K81A
0.081
NAD+
-
mutant enzyme K69A/K81A
0.128
NAD+
-
pH 10.4, 25°C, immobilized enzyme
0.13
NAD+
-
in 100 mM glycine-KCl-KOH buffer (pH 10.4)
0.16
NAD+
-
mutant enzyme K69A
0.17
NAD+
-
wild-type enzyme
0.234
NAD+
-
pH 10.4, 25°C, native enzyme
0.017
NADH
-
mutant enzyme K69A/K81A
0.026
NADH
-
mutant enzyme K81A
0.051
NADH
-
mutant enzyme K69A
0.083
NADH
-
wild-type enzyme
78
NH3
-
-
0.052
phenylpyruvate
-
mutant enzyme K81A
0.06
phenylpyruvate
-
wild-type enzyme
0.065
phenylpyruvate
-
wild-type enzyme
0.37
phenylpyruvate
-
25°C, pH 10.4
7
phenylpyruvate
-
mutant enzyme K69A/K81A
8.3
phenylpyruvate
-
mutant enzyme K69A
23
phenylpyruvate
-
mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q
1.4.1.20 phenylalanine dehydrogenase
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