1.4.1.20: phenylalanine dehydrogenase

This is an abbreviated version!
For detailed information about phenylalanine dehydrogenase, go to the full flat file.

Reaction

Synonyms

dehydrogenase, phenylalanine, L-PheDH, L-phenylalanine dehydrogenase, L-phenylalanine:NAD+ oxidoreductase, deaminating, PDH, PHD, PheDH, phenylalanine dehydrogenase, recombinant PheDH, recombinant phenylalanine dehydrogenase

ECTree

     1 Oxidoreductases

         1.4 Acting on the CH-NH₂ group of donors

             1.4.1 With NAD⁺ or NADP⁺ as acceptor

                1.4.1.20 phenylalanine dehydrogenase

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Results	in table
7	Activating Compound
41	AA Sequence
17	Application
1	CAS Registry Number
21	Cloned(Commentary)
85	Cofactor
7	Crystallization (Commentary)
1	General Information
8	General Stability
60	Inhibitors
38	kcat/KM [mM/s]
15	Ki Value [mM]
177	KM Value [mM]
3	Localization
1	Metals/Ions
32	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
6	Organic Solvent Stability
82	Organism
5	Pathway
4	PDB ID
27	pH Optimum
3	pH Range
10	pH Stability
1	Posttranslational Modification
49	Protein Variants
24	Purification (Commentary)
4	Reaction
3	Reaction Type
70	Reference
2	Source Tissue
18	Specific Activity [micromol/min/mg]
4	Storage Stability
228	Substrates and Products (Substrate)
25	Subunits
33	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
6	Temperature Range [°C]
30	Temperature Stability [°C]
82	Turnover Number [1/s]

pH Stability

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pH Stability on EC 1.4.1.20 - phenylalanine dehydrogenase

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10.4

Sporosarcina ureae

-

native enzyme, 15 min, no activity remaining at 30-45°C

723965

11.3

Lysinibacillus sphaericus

-

30°C, 1 h, stable

349634

4

Lysinibacillus sphaericus

-

30°C, 1 h, less than 20% loss of activity

349634

6 - 10.5

Fictibacillus nanhaiensis

A0A1B1PGG4

without any stabilizer, all samples except at pH 9.5, 10.0 and 6.0 maintain over 100% activity after being pre-treated in buffers ranging from pH 7.0-10.5 for 1 h at 30°C. The enzyme keeps more than 100% of its maximal activity after 1 h at 40°C (pH 7.0-9.0). Furthermore, it is stable under weak alkaline conditions (pH 7.0-9.0) after being pre-treated for 1 h at 50°C

743108

7.5

Sporosarcina ureae

-

native enzyme, 15 min, 96% activity remaining at 30-45°C

723965

8 - 10.4

Lysinibacillus sphaericus

-

in pH 8.0 buffer with either 10% methanol or 10% ethanol L307V PheDH retains almost 85% original activity after 7 days, therafter activity continues to decrease gradually, most rapidly in pH 10.4 buffer with 10% ethanol, wild type PheDH is similarly stable, mutant N145A PheDH shows 68% retention of activity after 7 days at pH 8.0 with 10% methanol

686436

8 - 12.5

Microbacterium sp.

-

30°C, 30 min, stable

349650

9

3 entries

9

Lysinibacillus sphaericus

-

30°C, 1 h, stable

349634

9

Sporosarcina ureae

-

30°C, 1 h, stable

349634

9

Sporosarcina ureae

-

native enzyme, 10 min, 75% activity remaining at 40°C, no activity at 50°C

723965