1.4.1.3: glutamate dehydrogenase [NAD(P)+] This is an abbreviated version! For detailed information about glutamate dehydrogenase [NAD(P)+], go to the full flat file .
Reaction
L-glutamate +
H2O +
NAD(P)+ =
2-oxoglutarate +
NH3 +
NAD(P)H +
H+
Synonyms At5g07440, At5g18170, dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate)), dual-coenzyme specific glutamate dehydrogenase, GDH, gdh-1, GDH1, GDH2, GDH3, GdhA, gdhA_1, GDHB, GDHII, GLDH, GLUD1, GLUD2, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamic acid dehydrogenase, glutamic dehydrogenase, hGDH1, hGDH2, hGLUD1, hGLUD2, housekeeping glutamate dehydrogenase, L-glutamate dehydrogenase, L-glutamic acid dehydrogenase, Legdh1, Membrane protein 50, MP50, NAD(P)+-dependent glutamate dehydrogenase, NAD(P)-dependent GDH, NAD(P)-dependent glutamate dehydrogenase, NAD(P)-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NAD(P)H-utilizing glutamate dehydrogenase, NADH-GDH, NADH-glutamate dehydrogenase, TTC1211, TTC1212, TtGDH
ECTree
General Stability
General Stability on EC 1.4.1.3 - glutamate dehydrogenase [NAD(P)+]
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2-mercaptoethanol stabilizes
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absolutely stable at 4°C or at room temperature for at least 6 months
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freeze-thawing decreases activity
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glycerol, 20% v/v, protects against cold inactivation
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glycerol, 5-20% v/v, stabilizes
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glyoxyl agarose-GDH immobilization is more stable than CNBr-agarose preparations or than the soluble enzyme, glyoxyl agarose derivative, prepared at 4°C for 15 min, immobilizes around 50% of the enzyme, but the enzyme retains almost full activity after the immobilization
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guanidine-dependent inactivation of the enzyme at 20°C is irreversible above 1.5 M guanidine hydrochloride
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SDS: 0.1% v/v, no loss of activity after 12 h, 0.5%, half-life 5 h
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strong dependence on high salt concentrations for stability
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urea: 4 M, 10% loss of activity after 12 h, 7.5 M, half-life 9 h
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