Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
160000
-
mutant enzyme H454Y, gel filtration
240000 - 260000
-
sucrose density gradient sedimentation
250000
-
liver enzyme, sedimentation velocity, light scattering
266000 - 269000
-
gel filtration
315000
recombinant His-tagged binary complex TtGDH, gel filtration
320000
-
enzyme from hibernating animals, gel filtration
335000
-
enzyme from euthermic animals, gel filtration
40500
-
6 * 40500, SDS-PAGE
46078
x * 46078, calculated from sequence
47040
6 * 47040, calculated from sequence, the natural enzyme was purified only as a hexameric form, whereas the recombinant enzyme was purified as both monomeric and hexameric forms. Only the enzyme in a hexameric form is active. Upon heat treatment (70°C for 15 min), the inactive monomeric form of the recombinant enzyme is at least partially associated with the hexameric form
47122
x * 47122, calculated from sequence
49000
-
6 * 49000, SDS-PAGE
50000
-
6 * 50000, membrane-bound liver enzyme, SDS-PAGE
52000
-
4 * 52000, SDS-PAGE
53900
-
6 * 53900, sedimentation equilibrium of enzyme treated with 6 M guanidinium and 0.5% 2-mercaptoethanol
54000
-
6 * 54000, SDS-PAGE
57500
-
x * 57500, recombinant enzyme, SDS-PAGE
59500
-
6 * 59500, enzyme from both euthermic and hibernating animals
60000
-
native gradient polyacryamide gel electrophoresis, 6 * 60000 Da
61000
-
8 * 61000, or aggregate of two tetramers, SDS-PAGE
270000
-
-
270000
-
gel filtration, sucrose density gradient centrifugation
270000
-
recombinant enzyme, only hexameric form is enzymatically active, gel filtration
284000
gel filtration
284000
-
recombinant and native enzyme, gel filtration
290000
-
gel filtration
290000
-
hig- and low-activity form, gel filtration
300000 - 350000
-
liver, light scattering
300000 - 350000
-
liver, sedimentation velocity
300000 - 350000
-
liver, sedimentation equilibrium
300000 - 350000
dogfish
-
liver, sedimentation equilibrium
300000 - 350000
-
liver, sedimentation equilibrium
300000 - 350000
-
isoenzymes GDH1, GDH2, disc gel electrophoresis
300000 - 350000
-
liver, light scattering, sedimentation equilibrium
300000 - 350000
-
polyacrylamide disc gel electrophoresis
330000
-
membrane-bound liver enzyme, velocity sedimentation on sucrose density gradient
330000
-
wild-type enzyme and mutant enzymes K333L, K337L, K344L, K346L, S445L and G446L, gel filtration
331000
-
gel filtration
331000
recombinant His-tagged ternary complex TtGDH-APRTh, gel filtration
333000
-
-
333000
-
sedimentation equilibrium
340000
-
gel filtration
44000
-
4 * 44000 + 2 * 46000, isoenzyme GDH2, SDS-PAGE
44000
-
6 * 44000, SDS-PAGE
45000
-
6 * 45000, SDS-PAGE
45000
-
x * 45000, SDS-PAGE
46000
-
6 * 46000, SDS-PAGE
46000
-
6 * 46000, SDS-PAGE
46000
-
4 * 44000 + 2 * 46000, isoenzyme GDH2, SDS-PAGE
46000
-
6 * 46000, recombinant enzyme, SDS-PAGE
47300
-
6 * 47300, recombinant enzyme, second peak in gel filtration corresponding to catalytically inactive monomer, SDS-PAGE
47300
6 * 47300, SDS-PAGE, the natural enzyme was purified only as a hexameric form, whereas the recombinant enzyme was purified as both monomeric and hexameric forms. Only the enzyme in a hexameric form is active. Upon heat treatment (70°C for 15 min), the inactive monomeric form of the recombinant enzyme is at least partially associated with the hexameric form
48000
-
6 * 48000, SDS-PAGE
48000
-
6 * 48000, SDS-PAGE
56000
-
6 * 56000, SDS-PAGE
56000
-
x * 56000, enzymes from mitochondria and endoplasmic reticulum
56000
-
6 * 56000, mutant enzyme H454Y
56000
-
6 * 56000, wild-type enzyme and mutant enzymes K333L, K337L, K344L, K346L, S445L and G446L
56500
-
6 * 56500, hGDH1, SDS-PAGE
56500
-
6 * 56500, hGDH2, SDS-PAGE
57000
-
x * 57000, SDS-PAGE
57000
-
6 * 57000, SDS-PAGE