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1.4.1.9: leucine dehydrogenase

This is an abbreviated version!
For detailed information about leucine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.9

Reaction

L-leucine
+
H2O
+
NAD+
=
4-methyl-2-oxopentanoate
+
NH3
+
NADH
+
H+

Synonyms

BCD, dehydrogenase, leucine, L-leucine dehydrogenase, L-leucine:NAD+ oxidoreductase, deaminating, LeuDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.9 leucine dehydrogenase

Storage Stability

Storage Stability on EC 1.4.1.9 - leucine dehydrogenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% glycerol, stable for 1 year
-
-20°C, stable for over 1 month
-
30°C, 2 months, 50% loss of activity in presence of 2.5 M NaCl
-
4°C, 5 mM tetrasodium EDTA solution in phosphate buffer, 0.1 M, pH 7.0, stable for at least 2 months
-
4°C, 50 mM potassium phosphate, 1 mM dithioerythritol, pH 7.5, 15% loss of activity after 2 months
-
4°C, buffer containing 0.02% azide, stable for more than 1 year
-
4°C, buffer containing 0.02% sodium azide, stable for more than 1 year
-
4°C, purified enzyme without any stabilizer, 1 month, barely changed activity
-
4°C, stable for at least 1 month
-
4°C, stable for over two years
-